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Isolation and Proteomic Analysis of a Chlamydomonas reinhardtii Mutant with Enhanced Lipid Production by the Gamma Irradiation Method
- Title
- Isolation and Proteomic Analysis of a Chlamydomonas reinhardtii Mutant with Enhanced Lipid Production by the Gamma Irradiation Method
- Authors
- Baek, Jaewon; Choi, Jong-il; Park, Hyun; Lim, Sangyong; Park, Si Jae
- Ewha Authors
- 박시재
- SCOPUS Author ID
- 박시재
- Issue Date
- 2016
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- ISSN
- 1017-7825
1738-8872
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY vol. 26, no. 12, pp. 2066 - 2075
- Keywords
- Chlamydomonas reinhardtii; lipid content; mutant; proteome
- Publisher
- KOREAN SOC MICROBIOLOGY &
BIOTECHNOLOGY
- Indexed
- SCIE; SCOPUS; KCI
- Document Type
- Article
- Abstract
- In this study, an enhanced lipid-producing mutant strain of the microalga Chlamydomonas reinhardtii was developed by gamma irradiation. To induce the mutation, C. reinhardtii was gamma irradiated at a dose of 400 Gy. After irradiation, the surviving cells were stained with Nile red. The mutant (Cr-4013) accumulating 20% more lipid than the wild type was selected. Thin-layer chromatography revealed the triglyceride and free fatty acid contents to be markedly increased in Cr-4013. The major fatty acids identified were palmitic acid, oleic acid, linoleic acid, and linolenic acid. Random amplified polymeric DNA analysis showed partial genetic modifications in Cr-4013. To ascertain the changes of protein expression in the mutant strain, two-dimensional electrophoresis was conducted. These results showed that gamma radiation could be used for the development of efficient microalgal strains for lipid production.
- DOI
- 10.4014/jmb.1605.05057
- Appears in Collections:
- 공과대학 > 화공신소재공학과 > Journal papers
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