Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박시재 | * |
dc.date.accessioned | 2019-11-19T16:30:28Z | - |
dc.date.available | 2019-11-19T16:30:28Z | - |
dc.date.issued | 2016 | * |
dc.identifier.issn | 1017-7825 | * |
dc.identifier.issn | 1738-8872 | * |
dc.identifier.other | OAK-25938 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/251933 | - |
dc.description.abstract | In this study, an enhanced lipid-producing mutant strain of the microalga Chlamydomonas reinhardtii was developed by gamma irradiation. To induce the mutation, C. reinhardtii was gamma irradiated at a dose of 400 Gy. After irradiation, the surviving cells were stained with Nile red. The mutant (Cr-4013) accumulating 20% more lipid than the wild type was selected. Thin-layer chromatography revealed the triglyceride and free fatty acid contents to be markedly increased in Cr-4013. The major fatty acids identified were palmitic acid, oleic acid, linoleic acid, and linolenic acid. Random amplified polymeric DNA analysis showed partial genetic modifications in Cr-4013. To ascertain the changes of protein expression in the mutant strain, two-dimensional electrophoresis was conducted. These results showed that gamma radiation could be used for the development of efficient microalgal strains for lipid production. | * |
dc.language | English | * |
dc.publisher | KOREAN SOC MICROBIOLOGY & | * |
dc.publisher | BIOTECHNOLOGY | * |
dc.subject | Chlamydomonas reinhardtii | * |
dc.subject | lipid content | * |
dc.subject | mutant | * |
dc.subject | proteome | * |
dc.title | Isolation and Proteomic Analysis of a Chlamydomonas reinhardtii Mutant with Enhanced Lipid Production by the Gamma Irradiation Method | * |
dc.type | Article | * |
dc.relation.issue | 12 | * |
dc.relation.volume | 26 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 2066 | * |
dc.relation.lastpage | 2075 | * |
dc.relation.journaltitle | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | * |
dc.identifier.doi | 10.4014/jmb.1605.05057 | * |
dc.identifier.wosid | WOS:000394576400007 | * |
dc.author.google | Baek, Jaewon | * |
dc.author.google | Choi, Jong-il | * |
dc.author.google | Park, Hyun | * |
dc.author.google | Lim, Sangyong | * |
dc.author.google | Park, Si Jae | * |
dc.contributor.scopusid | 박시재(57191670770) | * |
dc.date.modifydate | 20240322131024 | * |