View : 17 Download: 0
Microbial compositional changes in broiler chicken cecal contents from birds challenged with different Salmonella vaccine candidate strains
- Microbial compositional changes in broiler chicken cecal contents from birds challenged with different Salmonella vaccine candidate strains
- Park, Si Hong; Kim, Sun Ae; Rubinelli, Peter M.; Roto, Stephanie M.; Ricke, Steven C.
- Ewha Authors
- Issue Date
- Journal Title
- VACCINE vol. 35, no. 24, pp. 3204 - 3208
- Salmonella; Vaccine; Poultry; Microbiome; Sequencing
- ELSEVIER SCI LTD
- SCI; SCIE; SCOPUS
- Document Type
- Previously, we constructed and characterized the vaccine efficacy of Salmonella Typhimurium mutant strains in poultry with either inducible mviN expression (P-BAD-mviN) or methionine auxotrophy (Delta Delta metRmetD). The aim of the present study was to assess potential impact of these Salmonella vaccine strains on the cecal microbiota using a next generation sequencing (NGS). The cecal microbial community obtained from unvaccinated (group 1) and vaccinated chickens (group 2, vaccinated with PBAD-mviN; group 3, vaccinated with wild type; group 4, vaccinated with Delta Delta metRmetD) were subjected to micro-biome sequencing analysis with an Illumina MiSeq platform. The NGS microbiome analysis of chicken ceca revealed considerable changes in microbial composition in the presence of the different vaccine strains and exhibited detectable patterns of distinctive clustering among the respective groups (the R value of unweighted PCoA plot was 0.68). The present study indicates that different S. Typhimurium vaccine strains can differentially influence the microbiota of the ceca in terms of presence but not in the relative abundance of microbiota. (C) 2017 Elsevier Ltd. All rights reserved.
- Appears in Collections:
- 엘텍공과대학 > 식품공학전공 > Journal papers
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.