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Microbial compositional changes in broiler chicken cecal contents from birds challenged with different Salmonella vaccine candidate strains

Title
Microbial compositional changes in broiler chicken cecal contents from birds challenged with different Salmonella vaccine candidate strains
Authors
Park, Si HongKim, Sun AeRubinelli, Peter M.Roto, Stephanie M.Ricke, Steven C.
Ewha Authors
김선애
Issue Date
2017
Journal Title
VACCINE
ISSN
0264-410XJCR Link

1873-2518JCR Link
Citation
VACCINE vol. 35, no. 24, pp. 3204 - 3208
Keywords
SalmonellaVaccinePoultryMicrobiomeSequencing
Publisher
ELSEVIER SCI LTD
Indexed
SCI; SCIE; SCOPUS WOS
Document Type
Article
Abstract
Previously, we constructed and characterized the vaccine efficacy of Salmonella Typhimurium mutant strains in poultry with either inducible mviN expression (P-BAD-mviN) or methionine auxotrophy (Delta Delta metRmetD). The aim of the present study was to assess potential impact of these Salmonella vaccine strains on the cecal microbiota using a next generation sequencing (NGS). The cecal microbial community obtained from unvaccinated (group 1) and vaccinated chickens (group 2, vaccinated with PBAD-mviN; group 3, vaccinated with wild type; group 4, vaccinated with Delta Delta metRmetD) were subjected to micro-biome sequencing analysis with an Illumina MiSeq platform. The NGS microbiome analysis of chicken ceca revealed considerable changes in microbial composition in the presence of the different vaccine strains and exhibited detectable patterns of distinctive clustering among the respective groups (the R value of unweighted PCoA plot was 0.68). The present study indicates that different S. Typhimurium vaccine strains can differentially influence the microbiota of the ceca in terms of presence but not in the relative abundance of microbiota. (C) 2017 Elsevier Ltd. All rights reserved.
DOI
10.1016/j.vaccine.2017.04.073
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엘텍공과대학 > 식품공학전공 > Journal papers
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