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Therapeutic effects of three human-derived materials in a mouse corneal alkali burn model*

Title
Therapeutic effects of three human-derived materials in a mouse corneal alkali burn model*
Authors
Han K.E.Park M.H.Kong K.H.Choi E.Choi K.-R.Jun R.M.
Ewha Authors
최규룡박미혜전루민공경혜한경은최은오
SCOPUS Author ID
최규룡scopus; 박미혜scopus; 전루민scopus
Issue Date
2019
Journal Title
Cutaneous and Ocular Toxicology
ISSN
1556-9527JCR Link
Citation
Cutaneous and Ocular Toxicology vol. 38, no. 4, pp. 315 - 321
Keywords
Alkali injuryamniotic membrane suspensionperipheral blood serumumbilical cord serum
Publisher
Taylor and Francis Ltd
Indexed
SCIE; SCOPUS scopus
Document Type
Article
Abstract
Purpose: To compare the therapeutic effects of human derivatives in a mouse alkali burn model. Methods: The right eyes of mice were injured using NaOH. After alkali injury, one of the following agents was topically administered for 7 d: human amniotic membrane (hAM) suspension, human umbilical cord serum (hUCS), and human peripheral blood serum (hPBS), or saline. The epithelial defect areas on days 1, 2, and 3 degrees of opacity on days 2, 3, and 7, and corneal neovascularization (NV) areas on day 7 were evaluated. Histologic examination and mRNA expression levels of tumour necrosis factor (TNF)-α, interleukin (IL)-6, vascular endothelial growth factor (VEGF), matrix metalloproteinase (MMP)-2, MMP-8, and MMP-9 were also evaluated on day 7. Results: The epithelial defect areas in the hUCS group were smaller than those in the control and hPBS groups on day 3 (p <.05, respectively). The epithelial defect areas in the hAM suspension group showed smaller than those in the control and hPBS groups on days 1 and 2 (p <.05, respectively). The degrees of opacity were lower in all treatment groups than that of the saline control group on day 7 (p <.05, respectively). Corneal NV areas were not different among groups on day 7 (p = 0.20). The expression levels of TNF-α, IL-6, MMP-8, and MMP-9 mRNA and the infiltration of the inflammatory cells in all treatment groups were lesser than those in the control group on day 7 (p< .05, respectively). Conclusions: All treatments reduced inflammatory reactions and corneal opacity development. Corneal reepithelialization was faster in the hUCS group. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.
DOI
10.1080/15569527.2019.1573244
Appears in Collections:
의과대학 > 의학과 > Journal papers
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