Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 차선신 | * |
dc.date.accessioned | 2019-10-29T16:30:20Z | - |
dc.date.available | 2019-10-29T16:30:20Z | - |
dc.date.issued | 2019 | * |
dc.identifier.issn | 0066-4804 | * |
dc.identifier.issn | 1098-6596 | * |
dc.identifier.other | OAK-25566 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/251631 | - |
dc.description.abstract | ACC-1 is a plasmid-encoded class C beta-lactamase identified in clinical isolates of Klebsiella pneumoniae, Proteus mirabilis, Salmonella enterica, and Escherichia coli. ACC-1-producing bacteria are susceptible to cefoxitin, whereas they are resistant to oxyimino cephalosporins. Here, we depict crystal structures of apo ACC-1, adenylylated ACC-1, and acylated ACC-1 complexed with cefotaxime and cefoxitin. ACC-1 has noteworthy structural alterations in the R2 loop, the Omega loop, and the Phe119 loop located along the active-site rim. The adenylate covalently bonded to the nucleophilic serine reveals a tetrahedral phosphorus mimicking the deacylation transition state. Cefotaxime in ACC-1 has a proper conformation for the substrate-assisted catalysis in that its C-4 carboxylate and N-5 nitrogen are adequately located to facilitate the deacylation reaction. In contrast, cefoxitin in ACC-1 has a distinct conformation, in which those functional groups cannot contribute to catalysis. Furthermore, the orientation of the deacylating water relative to the acyl carbonyl group in ACC-1 is unfavorable for nucleophilic attack. | * |
dc.language | English | * |
dc.publisher | AMER SOC MICROBIOLOGY | * |
dc.subject | crystal structures | * |
dc.subject | ACC-1 class C beta-lactamase | * |
dc.subject | adenylylation | * |
dc.subject | acyl-enzyme complex | * |
dc.subject | cefotaxime | * |
dc.subject | cefoxitin | * |
dc.title | Structural Insights into Catalytic Relevances of Substrate Poses in ACC-1 | * |
dc.type | Article | * |
dc.relation.issue | 11 | * |
dc.relation.volume | 63 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | * |
dc.identifier.doi | 10.1128/AAC.01411-19 | * |
dc.identifier.wosid | WOS:000492306300002 | * |
dc.identifier.scopusid | 2-s2.0-85073765114 | * |
dc.author.google | Bae, Da-Woon | * |
dc.author.google | Jung, Ye-Eun | * |
dc.author.google | An, Young Jun | * |
dc.author.google | Na, Jung-Hyun | * |
dc.author.google | Cha, Sun-Shin | * |
dc.contributor.scopusid | 차선신(7201864593) | * |
dc.date.modifydate | 20240429134916 | * |