Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 장준 | * |
dc.date.accessioned | 2019-10-08T16:30:02Z | - |
dc.date.available | 2019-10-08T16:30:02Z | - |
dc.date.issued | 2019 | * |
dc.identifier.issn | 1932-6203 | * |
dc.identifier.other | OAK-25441 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/251597 | - |
dc.description.abstract | Middle East respiratory syndrome coronavirus (MERS-CoV) causes an acute and severe lower respiratory illness as well as vomiting, diarrhea, and renal failure. Because no licensed MERS-CoV vaccines are currently available, preventive and therapeutic measures are urgently needed. The surface spike (S) glycoprotein of MERS-CoV, which binds to the cellular receptor dipeptidyl peptidase 4 (DPP4), is considered as a major target for MERS-CoV vaccine development. Here, we designed recombinant replication-deficient adenovirus-based vaccines expressing the N-terminal domain (rAd/NTD) and receptor-binding domain (rAd/RBD) of the MERS-CoV S1 subunit and full-length Spike protein (rAd/Spike). We found that immunization with candidate vaccines via intranasal route induced S1-specific IgG antibodies and neutralizing antibodies against MERS spike pseudotyped virus. Especially, rAd/Spike induced the highest neutralizing antibody titer and the strongest cytokine-induced T cell responses among the three candidate vaccines. To compare the immune responses induced by different administration routes, rAd/Spike was administered via intranasal, sublingual, or intramuscular route. All these administration routes exhibited neutralizing effects in the serum. MERS-CoV-specific neutralizing IgA antibodies in the bronchoalveolar lavage fluid were only induced by intranasal and sublingual administration but not by intramuscular administration. Intranasal administration with rAd/Spike also created resident memory CD8 T cells in the airway and lung parenchyma. Taken together, our results showed that both the humoral and cellular immune responses are highly induced by rAd/Spike administration, suggesting that rAd/Spike may confer protection against MERS-CoV infection. | * |
dc.language | English | * |
dc.publisher | PUBLIC LIBRARY SCIENCE | * |
dc.title | Superior immune responses induced by intranasal immunization with recombinant adenovirus-based vaccine expressing full-length Spike protein of Middle East respiratory syndrome coronavirus | * |
dc.type | Article | * |
dc.relation.issue | 7 | * |
dc.relation.volume | 14 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | PLOS ONE | * |
dc.identifier.doi | 10.1371/journal.pone.0220196 | * |
dc.identifier.wosid | WOS:000484974100054 | * |
dc.author.google | Kim, Myung Hee | * |
dc.author.google | Kim, Hyun Jik | * |
dc.author.google | Chang, Jun | * |
dc.contributor.scopusid | 장준(8735999100) | * |
dc.date.modifydate | 20231120165756 | * |