View : 47 Download: 7

Full metadata record

DC Field Value Language
dc.contributor.author김진흥-
dc.date.accessioned2019-07-29T16:30:12Z-
dc.date.available2019-07-29T16:30:12Z-
dc.date.issued2018-
dc.identifier.issn2470-1343-
dc.identifier.otherOAK-25128-
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/250217-
dc.description.abstractInvestigation into the interactions between biomolecules DNA/RNA and carbon nanomaterials is very important for applications in bioassays and bioanalysis. Graphene and graphene oxide (GO) have been successfully adopted by exploiting the binding affinity difference between single-stranded oligonucleotides (ssDNA) and double- stranded oligonucleotides (dsDNA) to graphene sheets. In this work, we describe the electrochemical DNA oxidation with [Ru(bpy)(3)](2+) to understand the interaction between dsDNA (and corresponding ssDNA) and reduced graphene oxide (rGO). The electrochemical oxidation rate of guanine bases of ssDNA bound to rGO by electrochemically generated [Ru(bpy)(3)](3+) was much slower than those unbound to rGO. Our study revealed that ssDNA constrained on rGO was significantly protected from the electron transfer to [Ru(bpy)(3)](3+) because of pi,pi-stacking interaction between nucleobases and rGO. On the other hand, the oxidation rates of 11-, 20-, and 27-mer dsDNA bound to rGO increased relative to those of dsDNA alone, demonstrating that the guanine bases of dsDNA on the interaction with rGO became more accessible to [Ru(bpy)(3)](3+). Our electrochemical data illustrated that dsDNA could be totally or partially dehybridized and bind to rGO to form ssDNA/rGO. Furthermore, absorption, circular dichroism spectra, and fluorescence measurements of ethidium bromide using ssDNA and dsDNA with rGO supported the dehybridization of dsDNA in the presence of rGO.-
dc.languageEnglish-
dc.publisherAMER CHEMICAL SOC-
dc.titleReduced Graphene Oxide-Oligonucleotide Interfaces: Understanding Based on Electrochemical Oxidation of Guanines-
dc.typeArticle-
dc.relation.issue11-
dc.relation.volume3-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage15464-
dc.relation.lastpage15470-
dc.relation.journaltitleACS OMEGA-
dc.identifier.doi10.1021/acsomega.8b02063-
dc.identifier.wosidWOS:000451992500097-
dc.author.googleTikum, Anjong Florence-
dc.author.googleKo, Jeong Won-
dc.author.googleKim, Soojin-
dc.author.googleKim, Jinheung-
dc.contributor.scopusid김진흥(8580015800)-
dc.date.modifydate20190729153300-


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE