Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 김진흥 | - |
dc.date.accessioned | 2019-07-29T16:30:12Z | - |
dc.date.available | 2019-07-29T16:30:12Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 2470-1343 | - |
dc.identifier.other | OAK-25128 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/250217 | - |
dc.description.abstract | Investigation into the interactions between biomolecules DNA/RNA and carbon nanomaterials is very important for applications in bioassays and bioanalysis. Graphene and graphene oxide (GO) have been successfully adopted by exploiting the binding affinity difference between single-stranded oligonucleotides (ssDNA) and double- stranded oligonucleotides (dsDNA) to graphene sheets. In this work, we describe the electrochemical DNA oxidation with [Ru(bpy)(3)](2+) to understand the interaction between dsDNA (and corresponding ssDNA) and reduced graphene oxide (rGO). The electrochemical oxidation rate of guanine bases of ssDNA bound to rGO by electrochemically generated [Ru(bpy)(3)](3+) was much slower than those unbound to rGO. Our study revealed that ssDNA constrained on rGO was significantly protected from the electron transfer to [Ru(bpy)(3)](3+) because of pi,pi-stacking interaction between nucleobases and rGO. On the other hand, the oxidation rates of 11-, 20-, and 27-mer dsDNA bound to rGO increased relative to those of dsDNA alone, demonstrating that the guanine bases of dsDNA on the interaction with rGO became more accessible to [Ru(bpy)(3)](3+). Our electrochemical data illustrated that dsDNA could be totally or partially dehybridized and bind to rGO to form ssDNA/rGO. Furthermore, absorption, circular dichroism spectra, and fluorescence measurements of ethidium bromide using ssDNA and dsDNA with rGO supported the dehybridization of dsDNA in the presence of rGO. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Reduced Graphene Oxide-Oligonucleotide Interfaces: Understanding Based on Electrochemical Oxidation of Guanines | - |
dc.type | Article | - |
dc.relation.issue | 11 | - |
dc.relation.volume | 3 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 15464 | - |
dc.relation.lastpage | 15470 | - |
dc.relation.journaltitle | ACS OMEGA | - |
dc.identifier.doi | 10.1021/acsomega.8b02063 | - |
dc.identifier.wosid | WOS:000451992500097 | - |
dc.author.google | Tikum, Anjong Florence | - |
dc.author.google | Ko, Jeong Won | - |
dc.author.google | Kim, Soojin | - |
dc.author.google | Kim, Jinheung | - |
dc.contributor.scopusid | 김진흥(8580015800) | - |
dc.date.modifydate | 20230201114415 | - |