Design and shelf stability assessment of bacterial agents for simultaneous removal of methane and odors

Abstract

Two types of solid bacterial agents for the simultaneous removal of methane and odor were designed using humic soil (De-MO-1) and the mixture of humic soil and tobermolite (De-MO-2) as biocarriers. The bacterial consortium, having the removability of methane and dimethyl sulfide (DMS), was immobilized in the biocarriers, and then stored at room temperature for 375days without additional treatment. Although the lag period, of which the incubation time required for removing methane and DMS, tended to increase over the storage period, the removability of methane and DMS was maintained during 375days in both bacterial agents. Key bacteria associated with the removal of methane and odors (Streptomyces, Promicromonospora, Paracoccus, Lysobacter, Sphingopyxis and Methylosystis) could keep their abundance during the storage period. The richness and evenness values of the bacterial communities in De-MO-1 and De-MO-2 ranged 4.89 similar to 6.50 and 0.89 similar to 0.98, respectively, indicating that high bacterial diversity was maintained during the storage period. The results suggest that De-MO-1 and De-MO-2, designed for the simultaneous removal of methane and odors, had shelf stabilities over one year.