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Structural basis for the selective addition of an oxygen atom to cyclic ketones by Baeyer-Villiger monooxygenase from Parvibaculum lavamentivorans
- Title
- Structural basis for the selective addition of an oxygen atom to cyclic ketones by Baeyer-Villiger monooxygenase from Parvibaculum lavamentivorans
- Authors
- Tien Duc Nguyen; Choi, Go-Eun; Gu, Do-Heon; Seo, Pil-Won; Kim, Ji-Won; Park, Jin-Byung; Kim, Jeong-Sun
- Ewha Authors
- 박진병
- SCOPUS Author ID
- 박진병
- Issue Date
- 2019
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- ISSN
- 0006-291X
1090-2104
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS vol. 512, no. 3, pp. 564 - 570
- Keywords
- Baeyer-villiger monooxygenase; BVMOParvi; BVMOOcean; Bulge
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Baeyer-Villiger monooxygenase (BVMO) catalyzes insertion of an oxygen atom into aliphatic or cyclic ketones with high regioselectivity. The BVMO5 from Parvibaculum lavamentivorans (BVMOParvi) and Oceanicola batsensis (BVMOOcean) are interesting because of their homologies, with >40% sequence identity, and reaction with the same cyclic ketones with a methyl moiety to give different products. The revealed BVMOParvi structure shows that BVMOParvi forms a two-domain structure like other BVMOs. It has two inserted residues, compared with BVMOOcean, that form a bulge near the bound flavin adenine dinucleotide in the active site. Furthermore, this bulge is linked to a nearby alpha-helix via a disulfide bond, probably restricting access of the bulky methyl group of the substrate to this bulge. Another sequence motif at the entrance of the active site (Ala-Ser in BVMOParvi, and Ser-Thr in BVMOOcean) allows a large volume in BVMOParvi These minute differences may discriminate a substrate orientation in both BVMOs from the initial substrate binding pocket to the final oxygenation site, resulting in the inserted oxygen atom being in different positions of the same substrate. (C) 2019 Elsevier Inc. All rights reserved.
- DOI
- 10.1016/j.bbrc.2019.03.114
- Appears in Collections:
- 공과대학 > 식품생명공학과 > Journal papers
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