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Rhodamine-based near-infrared probe for emission detection of ATP in lysosomes in living cells

Title
Rhodamine-based near-infrared probe for emission detection of ATP in lysosomes in living cells
Authors
Tikum A.F.Kim G.Nasirian A.Ko J.W.Yoon J.Kim J.
Ewha Authors
윤주영김진흥
SCOPUS Author ID
윤주영scopus; 김진흥scopus
Issue Date
2019
Journal Title
Sensors and Actuators, B: Chemical
ISSN
0925-4005JCR Link
Citation
Sensors and Actuators, B: Chemical vol. 292, pp. 40 - 47
Keywords
Adenosine triphosphateCell imagingFluorescence detectionFluorescent probe for ATPLysosome targeting
Publisher
Elsevier B.V.
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
A new near infrared fluorescent probe was designed and prepared to detect ATP and target lysosomes in cells. The non-fluorescent probe was turned on during the interaction with ATP, affording a 115-fold increase in fluorescence intensity. The colorless probe turned purple upon interaction with ATP. The probe displayed high sensitivity and selectivity toward ATP compared with other biomolecules, such as AMP, ADP, GMP, and CMP. Such significant spectroscopic changes derive from a structural change of the probe's spirocyclic moiety to its ring-opened form. This occurs through electrostatic and π,π-stacking interactions between the probe and ATP. This probe was also used in selective lysosome staining and for real-time ATP level monitoring. It exhibits great advantages in cell imaging studies, such as excellent photostability, low cytotoxicity, and good cell membrane permeability. © 2019 Elsevier B.V.
DOI
10.1016/j.snb.2019.04.112
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
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