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Pretreatment of kenaf (Hibiscus cannabinus L.) biomass feedstock for polyhydroxybutyrate (PHB) production and characterization

Title
Pretreatment of kenaf (Hibiscus cannabinus L.) biomass feedstock for polyhydroxybutyrate (PHB) production and characterization
Authors
Saratale, Rijuta GaneshSaratale, Ganesh DattatrayaCho, Si KyungKim, Dong SuGhodake, Gajanan S.Kadam, AvinashKumar, GopalakrishananBharagava, Ram NareshBanu, RajeshShin, Han Seung
Ewha Authors
김동수
SCOPUS Author ID
김동수scopus
Issue Date
2019
Journal Title
BIORESOURCE TECHNOLOGY
ISSN
0960-8524JCR Link

1873-2976JCR Link
Citation
BIORESOURCE TECHNOLOGY vol. 282, pp. 75 - 80
Keywords
Kenaf biomassGreen liquor pretreatmentPoly(3-hydroxybutyrate)Ralstonia eutropha ATCC 17699Enzymatic hydrolysis
Publisher
ELSEVIER SCI LTD
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Kenaf biomass (KB) was employed as feedstock for the synthesis of polyhydroxybutyrate (PHB) using Ralstonia eutropha to replace conventional petroleum-derived polymers. Various pretreatments followed by enzymatic saccharification were applied to release monomeric sugars from KB for PHB production. The effects of increasing concentration of Na2CO3 + Na2SO3 (NaC+ NaS) pretreated KB hydrolysates (20-40 g/L) on PHB production were investigated. NaC+ NaS pretreated KB hydrolysates (30 g/L) exhibited maximum 70.0% PHA accumulation, with PHB titers of 10.10 g/L and PHB yields of about 0.488 g/g of reducing sugar produced within 36 h of fermentation. PHA accumulation, PHB yield and R. eutropha growth performance using KB hydrolysates were found to be comparable with those of synthetic sugar mixture. Characterization of the produced PHB in terms of crystalline structure, and thermal properties was done using various analytical techniques and results coincide with standard PHB. Thus, green liquor pretreated KB hydrolysates deliver a promising and economically feasible carbon substrate for PHB production.
DOI
10.1016/j.biortech.2019.02.083
Appears in Collections:
엘텍공과대학 > 환경공학전공 > Journal papers
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