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Silver nanoparticles promote procoagulant activity of red blood cells: a potential risk of thrombosis in susceptible population

Title
Silver nanoparticles promote procoagulant activity of red blood cells: a potential risk of thrombosis in susceptible population
Authors
Bian, YiyingKim, KeunyoungThien NgoKim, InhoBae, Ok-NamLim, Kyung-MinChung, Jin-Ho
Ewha Authors
임경민
SCOPUS Author ID
임경민scopus
Issue Date
2019
Journal Title
PARTICLE AND FIBRE TOXICOLOGY
ISSN
1743-8977JCR Link
Citation
PARTICLE AND FIBRE TOXICOLOGY vol. 16
Keywords
Silver nanoparticles (AgNP)ToxicityThrombosisRed blood cells (RBCs)Cancer
Publisher
BMC
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Silver nanoparticles (AgNP) are widely used in medical practices owing to their distinct antibacterial, antiviral and anticancer activities. However, with increasing use of AgNP, concerns over its potential toxicity are also escalating. Here, we demonstrated the potential thrombotic effect of AgNP which was mediated by the procoagulant activity of red blood cells (RBCs). In freshly isolated human RBCs, AgNP, but not silver microparticles (AgMP), elicited morphological changes, phosphatidylserine (PS) exposure and microvesicles (MV) generation, the key indicators of procoagulant activity in RBCs at concentration ranges (ae 100 mu g/mL) that were free of significant hemolysis. In line with this, AgNP potentiated thrombin generation and adherence of RBCs to endothelial cells, while AgMP did not. Oxidative stress, intracellular calcium increase and ATP depletion were found to underlie the procoagulant effects of AgNP, which led to altered activity of membrane aminophospholipid translocases. These in vitro findings were well reproduced in rat in vivo, where intravenously exposure to AgNP promoted venous thrombosis significantly. Of note, RBCs isolated from cancer patients, who inherently convey the risk of thrombogenesis, were more sensitive to the procoagulant effects of AgNP. In addition, AgNP significantly potentiated the procoagulant effects of a chemotherapeutic drug, paclitaxel. Collectively, these results suggest that AgNP may have prothrombotic risks by promoting procoagulant activity of RBCs and caution shall be taken for its use in the population sensitive to thrombosis like cancer patients.
DOI
10.1186/s12989-019-0292-6
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약학대학 > 약학과 > Journal papers
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