Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 손아정 | * |
dc.contributor.author | 이은희 | * |
dc.date.accessioned | 2019-01-24T16:30:13Z | - |
dc.date.available | 2019-01-24T16:30:13Z | - |
dc.date.issued | 2019 | * |
dc.identifier.issn | 1385-8947 | * |
dc.identifier.other | OAK-24135 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/248248 | - |
dc.description.abstract | A highly sensitive fluorescence resonance energy transfer (FRET)-based quantum dot (QD)-Aptasensor was developed for microcystin-LR (MC-LR) detection in eutrophic water. The FRET-based QD-Aptasensor was able to achieve an analytical limit of detection of 10−4 μg/L MC-LR with selectivity among different congeners (microcystin-YR, microcystin-LY, microcystin-LW, microcystin-RR, microcystin-LF, microcystin-LA, and Nodularin). The performance of QD-Aptasensor was demonstrated using both laboratory grown cyanobacterial culture (Microcystis aeruginosa) and environmental water samples. For the laboratory culture, the intracellular MC-LR concentrations changed according to the cyanobacterial growth curve. The QD-Aptasensor detected MC-LR up to 12.7–15.8 μg/L-cyanobacterial culture at early stationary phase. For the environmental samples, the MC-LR was measured at cell densities of 2.7 × 108 and 6.6 × 1010 cells/L-water, which correspond to 1.0 and 7.2 μg MC-LR/L-water, respectively. For comparison, the laboratory culture and environmental samples were analyzed by the conventional enzyme-linked immuno-sorbent assay (ELISA). The MC-LR concentrations of FRET-based QD-Aptasensor showed a strong positive correlation with those of ELISA (Pearson correlation coefficient, r = 0.981 and r = 0.862) for the laboratory culture and environmental samples, respectively. © 2018 Elsevier B.V. | * |
dc.language | English | * |
dc.publisher | Elsevier B.V. | * |
dc.subject | Aptamer | * |
dc.subject | Cyanobacterial bloom | * |
dc.subject | Cyanotoxin | * |
dc.subject | Fluorescence resonance energy transfer (FRET) | * |
dc.subject | Quantum dots (QDs) | * |
dc.title | Fluorescence resonance energy transfer based quantum dot-Aptasensor for the selective detection of microcystin-LR in eutrophic water | * |
dc.type | Article | * |
dc.relation.volume | 359 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 1493 | * |
dc.relation.lastpage | 1501 | * |
dc.relation.journaltitle | Chemical Engineering Journal | * |
dc.identifier.doi | 10.1016/j.cej.2018.11.027 | * |
dc.identifier.wosid | WOS:000454137400143 | * |
dc.identifier.scopusid | 2-s2.0-85056357980 | * |
dc.author.google | Lee E.-H. | * |
dc.author.google | Son A. | * |
dc.contributor.scopusid | 손아정(23487252600) | * |
dc.contributor.scopusid | 이은희(55773710100;56141882500) | * |
dc.date.modifydate | 20240322131724 | * |