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Rhodamine derivatives bearing thiourea groups serve as fluorescent probes for selective detection of ATP in mitochondria and lysosomes

Title
Rhodamine derivatives bearing thiourea groups serve as fluorescent probes for selective detection of ATP in mitochondria and lysosomes
Authors
Swamy K.M.K.Eom S.Liu Y.Kim G.Lee D.Yoon J.
Ewha Authors
윤주영K.M.K Swamy
SCOPUS Author ID
윤주영scopus
Issue Date
2019
Journal Title
Sensors and Actuators, B: Chemical
ISSN
0925-4005JCR Link
Citation
Sensors and Actuators, B: Chemical vol. 281, pp. 350 - 358
Keywords
ATP probeFluorescent chemosensorMitochondria targetingRhodamineThiourea
Publisher
Elsevier B.V.
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
ATP functions as an energy source in all living things and it also plays key roles in a variety of important biological processes. Moreover, low levels of ATP are indicators of cardiovascular disease, Parkinson's disease and ischemia. As a result, the development of fluorescent probes that detect ATP selectively is a significant goal. In the current study, we synthesized two new rhodamine derivatives, 1 and 2, bearing thiourea groups, and assessed their abilities to serve as selective fluorescent and colorimetric probes for ATP. Thiourea groups were incorporated in 1 and 2 to act as hydrogen bonding donors to recognize tris-phosphate groups and induce photophysical property changing, spirocyclic ring opening. Indeed, we observed that solutions of these probes undergo a large fluorescent enhancement at 590 nm and a distinct colorimetric change from colorless to dark pink upon addition of ATP. Moreover, these probes can be used to image ATP in HeLa cells. Because it was found to locate mainly in mitochondria, 2 can be employed to image ATP in mitochondria. On the other hand, probe 1, bearing a lysosome directing morpholine group displays moderately selective localization in lysosomes and can be utilized to image ATP in lysosomes. Furthermore, both probes were shown to have extremely low cytotoxicities by using MTT assays. © 2018
DOI
10.1016/j.snb.2018.10.135
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
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