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Dehydrocostus lactone, a sesquiterpene from Saussurea lappa Clarke, suppresses allergic airway inflammation by binding to dimerized translationally controlled tumor protein
- Title
- Dehydrocostus lactone, a sesquiterpene from Saussurea lappa Clarke, suppresses allergic airway inflammation by binding to dimerized translationally controlled tumor protein
- Authors
- Pyun H.; Kang U.; Seo E.K.; Lee K.
- Ewha Authors
- 이경림; 서은경
- SCOPUS Author ID
- 이경림; 서은경
- Issue Date
- 2018
- Journal Title
- Phytomedicine
- ISSN
- 0944-7113
- Citation
- Phytomedicine vol. 43, pp. 46 - 54
- Keywords
- Allergic diseases; Dehydrocostus lactone; Dimerized TCTP; Histamine releasing factor; Saussurea lappa Clarke
- Publisher
- Elsevier GmbH
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Background: We previously reported that the biologically active form of histamine releasing factor (HRF) is dimerized translationally controlled tumor protein (dTCTP) which is involved in a number of allergic diseases. Hypothesis/purpose: Hoping that agents that modulate dTCTP may provide new therapeutic targets to allergic inflammatory diseases, we screened a library of natural products for substances that inhibit dTCTP. One such inhibitor we found was dehydrocostus lactone (DCL), a natural sesquiterpene present in rhizome of Saussurea lappa Clarke, the subject of this study. Methods: We evaluated the therapeutic efficacy of DCL in a mouse model of ovalbumin (OVA)-induced allergic airway inflammation, employing the ELISA system using BEAS-2B cells and splenocytes, and confirmed that DCL interacts with dTCTP using SPR assay. Results: DCL inhibited dTCTP-induced secretion of IL-8 in BEAS-2B cells. From kinetic analysis of dTCTP and DCL, we found that KD value was 5.33 ± 0.03 μM between dTCTP and DCL. DCL also significantly reduced inflammatory lung eosinophilia, type 2 cytokines in BALF, as well as OVA specific IgE and mucus production in a mouse model of ovalbumin induced allergy. Moreover, DCL suppressed NF-κB activation. Conclusion: DCL's therapeutic potential in allergic airway inflammation is based on its anti-inflammatory activity of suppressing the function of dTCTP. © 2018 Elsevier GmbH
- DOI
- 10.1016/j.phymed.2018.03.045
- Appears in Collections:
- 약학대학 > 약학과 > Journal papers
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