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Intracellular transformation rates of fatty acids are influenced by expression of the fatty acid transporter FadL in Escherichia coli cell membrane

Title
Intracellular transformation rates of fatty acids are influenced by expression of the fatty acid transporter FadL in Escherichia coli cell membrane
Authors
Jeon, Eun-YeongSong, Ji-WonCha, Hee-JeongLee, Sun-MeeLee, JinwonPark, Jin-Byung
Ewha Authors
박진병송지원
SCOPUS Author ID
박진병scopus
Issue Date
2018
Journal Title
JOURNAL OF BIOTECHNOLOGY
ISSN
0168-1656JCR Link

1873-4863JCR Link
Citation
JOURNAL OF BIOTECHNOLOGY vol. 281, pp. 161 - 167
Keywords
Long-chain fatty acid transporter FadLFatty acidsWhole cell biotransformationEscherichia coli
Publisher
ELSEVIER SCIENCE BV
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Fatty acids have a low permeability through the cell membrane. Therefore, the intracellular biotransformation of fatty acids can be slow due to supply limitations. The effects of expression level of the fatty acid transporter FadL in Escherichia coli on the biotransformations were investigated. The enhanced expression of FadL led to 5.5-fold increase of the maximum reaction rate V-max (i.e., 200 mu mol/min per g dry cells (200 U/g dry cells)) of the recombinant E. coli expressing a hydratase of Stenotrophomonas maltophilia in the periplasm with respect to hydration of oleic acid. The FadL expression level was also critical for oxidation of 12- and 10-hydroxyoctadecanoic acid by the recombinant E. coli expressing an alcohol dehydrogenase (ADH) of Micrococcus luteus. In addition, the multistep biotransformation of ricinoleic acid into the ester (i.e., (Z)-11-(heptanoyloxy) undec-9-enoic acid) by the recombinant E. coli expressing the ADH of M. luteus and a Baeyer-Villiger monooxygenase of Pseudomonas putida KT2440 was 2-fold increased to 40 U/g dry cells with expression of FadL to an appropriate level. The FadL expression level is one of the critical factors to determine whole-cell biotransformation rates of not only long chain fatty acids but also hydroxy fatty acids. This study may contribute to whole-cell biocatalyst engineering for biotransformation of hydrophobic substances.
DOI
10.1016/j.jbiotec.2018.07.019
Appears in Collections:
엘텍공과대학 > 식품공학전공 > Journal papers
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