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Increased expression of IL-33 in rosacea skin and UVB-irradiated and LL-37-treated HaCaT cells
- Title
- Increased expression of IL-33 in rosacea skin and UVB-irradiated and LL-37-treated HaCaT cells
- Authors
- Suhng, Eunah; Kim, Bo Hee; Choi, You Won; Choi, Hae Young; Cho, Hyunjin; Byun, Ji Yeon
- Ewha Authors
- 최혜영; 최유원; 변지연
- SCOPUS Author ID
- 최혜영; 최유원; 변지연
- Issue Date
- 2018
- Journal Title
- EXPERIMENTAL DERMATOLOGY
- ISSN
- 0906-6705
1600-0625
- Citation
- EXPERIMENTAL DERMATOLOGY vol. 27, no. 9, pp. 1023 - 1029
- Keywords
- cathelicidin; IL-33; LL-37; rosacea; ultraviolet B; vascular endothelial growth factor
- Publisher
- WILEY
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Rosacea is one of the most common dermatoses of adults. Although the detailed pathophysiology remains unknown, it is thought that rosacea is caused by a consistently aberrant, innate immune response, and that LL-37 plays an important role. However, involvement of the inflammatory cytokine IL-33 has not yet been studied. We explored the role played by IL-33 in the pathophysiology of rosacea. First, we immunohistochemically evaluated the expression of IL-33 and its receptor (ST2) in rosacea skin. Second, we exposed HaCaT cells to ultraviolet B (UVB) irradiation in the presence or absence of LL-37 and measured the expression of proinflammatory cytokines including IL-33. We also analysed VEGF (vascular endothelial growth factor) mRNA expression and protein release after costimulation of HaCaT cells by LL-37 and IL-33. Immunohistochemically, IL-33 expression was enhanced in the skin of rosacea patients, especially with erythematotelangiectatic subtype. In vitro, UVB and LL-37 synergistically increased mRNAs expression of proinflammatory cytokines, especially IL-33 and IL-1. IL-33 protein release was also synergistically increased by LL-37 and UVB treatment. LL-37 and IL-33 stimulated VEGF mRNA expression and VEGF release from HaCaT cells. Our findings suggest that rosacea skin with abundant LL-37 may robustly produce and release IL-33 when exposed to UV radiation. IL-33 may participate in the angiogenesis and vasodilation of rosacea skin by enhancing VEGF release.
- DOI
- 10.1111/exd.13702
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
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