View : 651 Download: 132
The quinone-based derivative, HMNQ induces apoptotic and autophagic cell death by modulating reactive oxygen species in cancer cells
- Title
- The quinone-based derivative, HMNQ induces apoptotic and autophagic cell death by modulating reactive oxygen species in cancer cells
- Authors
- Lee, Eun Byul; Cheon, Min Gyeong; Cui, Jun; Lee, Yoo Jin; Seo, Eun Kyoung; Jang, Ho Hee
- Ewha Authors
- 서은경
- SCOPUS Author ID
- 서은경
- Issue Date
- 2017
- Journal Title
- ONCOTARGET
- ISSN
- 1949-2553
- Citation
- ONCOTARGET vol. 8, no. 59, pp. 99637 - 99648
- Keywords
- anticancer drug; natural compound; reactive oxygen species (ROS); apoptosis; autophagy
- Publisher
- IMPACT JOURNALS LLC
- Indexed
- SCOPUS
- Document Type
- Article
- Abstract
- 8-Hydroxy-2-methoxy-1,4-naphthoquinone (HMNQ), a natural compound isolated from the bark of Juglans sinensis Dode, displays cytotoxic activity against various human cancer cells. However, the molecular mechanism of the anticancer effect is unclear. In this study, we examined the cytotoxic mechanism of HMNQ at the molecular level in human cancer cells. Cells were treated with HMNQ in a dose-or time-dependent manner. HMNQ treatment inhibited cell viability, colony formation and cell migration, indicating that HMNQ induced cancer cell death. HMNQ-treated cells resulted in apoptotic cell death through PARP-1 cleavage, Bax upregulation and Bcl-2 downregulation. HMNQ was also observed to induce autophagy by upregulating Beclin-1 and LC3. Furthermore, HMNQ induced reactive oxygen species (ROS) production, which was attenuated by the ROS scavengers, NAC and GSH. Finally, HMNQ increased expression of JNK phosphorylation and the JNK inhibitor SP600125 rescued HMNQ-induced cell death, suggesting that the cytotoxicity of HMNQ is mediated by the JNK signaling pathway. Taken together, our findings show that HMNQ exhibits anticancer activity through induction of ROS-mediated apoptosis and autophagy in human cancer cells. These data suggest the potential value of HMNQ as a natural anticancer drug.
- DOI
- 10.18632/oncotarget.21005
- Appears in Collections:
- 약학대학 > 약학과 > Journal papers
- Files in This Item:
-
The quinone-based derivative.pdf(8.67 MB)
Download
- Export
- RIS (EndNote)
- XLS (Excel)
- XML