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Characterization of a Whole-Cell Biotransformation Using a Constitutive Lysine Decarboxylase from Escherichia coli for the High-Level Production of Cadaverine from Industrial Grade l-Lysine

Title
Characterization of a Whole-Cell Biotransformation Using a Constitutive Lysine Decarboxylase from Escherichia coli for the High-Level Production of Cadaverine from Industrial Grade l-Lysine
Authors
Shin J.Joo J.C.Lee E.Hyun S.M.Kim H.J.Park S.J.Yang Y.-H.Park K.
Ewha Authors
박시재
SCOPUS Author ID
박시재scopus
Issue Date
2018
Journal Title
Applied Biochemistry and Biotechnology
ISSN
0273-2289JCR Link
Citation
Applied Biochemistry and Biotechnology vol. 185, no. 4, pp. 909 - 924
Keywords
Cadaverinel-LysineLysine decarboxylaseReusabilityWhole-cell biotransformation
Publisher
Humana Press Inc.
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Cadaverine is used for the synthesis of the novel bio-polyamides 54, 56, and 510. Here, we examine the feasibility of using a lysine decarboxylase (LdcC) from Escherichia coli for high-level production of cadaverine. After sequential optimization of whole-cell biotransformation conditions, recombinant E. coli-overexpressing LdcC (EcLdcC) could produce 1.0 M cadaverine from 1.2 M crude l-lysine solution after 9 h. EcLdcC retained a higher cadaverine yield after being reused 10 times at acidic and alkaline pH values than that of a recombinant E. coli strain overexpressing an inducible lysine decarboxylase (CadA), a conventional cadaverine producer (90 vs. 51% at pH 6 and 55 vs. 15% at pH 8). This study reveals that EcLdcC is a promising whole-cell biocatalyst for the bio-based production of cadaverine from industrial grade l-lysine in comparison to EcCadA. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.
DOI
10.1007/s12010-018-2696-4
Appears in Collections:
엘텍공과대학 > 화학신소재공학전공 > Journal papers
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