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Characterization of a Whole-Cell Biotransformation Using a Constitutive Lysine Decarboxylase from Escherichia coli for the High-Level Production of Cadaverine from Industrial Grade l-Lysine
- Title
- Characterization of a Whole-Cell Biotransformation Using a Constitutive Lysine Decarboxylase from Escherichia coli for the High-Level Production of Cadaverine from Industrial Grade l-Lysine
- Authors
- Shin J.; Joo J.C.; Lee E.; Hyun S.M.; Kim H.J.; Park S.J.; Yang Y.-H.; Park K.
- Ewha Authors
- 박시재
- SCOPUS Author ID
- 박시재
- Issue Date
- 2018
- Journal Title
- Applied Biochemistry and Biotechnology
- ISSN
- 0273-2289
- Citation
- Applied Biochemistry and Biotechnology vol. 185, no. 4, pp. 909 - 924
- Keywords
- Cadaverine; l-Lysine; Lysine decarboxylase; Reusability; Whole-cell biotransformation
- Publisher
- Humana Press Inc.
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Cadaverine is used for the synthesis of the novel bio-polyamides 54, 56, and 510. Here, we examine the feasibility of using a lysine decarboxylase (LdcC) from Escherichia coli for high-level production of cadaverine. After sequential optimization of whole-cell biotransformation conditions, recombinant E. coli-overexpressing LdcC (EcLdcC) could produce 1.0 M cadaverine from 1.2 M crude l-lysine solution after 9 h. EcLdcC retained a higher cadaverine yield after being reused 10 times at acidic and alkaline pH values than that of a recombinant E. coli strain overexpressing an inducible lysine decarboxylase (CadA), a conventional cadaverine producer (90 vs. 51% at pH 6 and 55 vs. 15% at pH 8). This study reveals that EcLdcC is a promising whole-cell biocatalyst for the bio-based production of cadaverine from industrial grade l-lysine in comparison to EcCadA. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.
- DOI
- 10.1007/s12010-018-2696-4
- Appears in Collections:
- 공과대학 > 화공신소재공학과 > Journal papers
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