Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박시재 | * |
dc.date.accessioned | 2018-11-21T16:30:48Z | - |
dc.date.available | 2018-11-21T16:30:48Z | - |
dc.date.issued | 2018 | * |
dc.identifier.issn | 0273-2289 | * |
dc.identifier.other | OAK-22074 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/246864 | - |
dc.description.abstract | Cadaverine is used for the synthesis of the novel bio-polyamides 54, 56, and 510. Here, we examine the feasibility of using a lysine decarboxylase (LdcC) from Escherichia coli for high-level production of cadaverine. After sequential optimization of whole-cell biotransformation conditions, recombinant E. coli-overexpressing LdcC (EcLdcC) could produce 1.0 M cadaverine from 1.2 M crude l-lysine solution after 9 h. EcLdcC retained a higher cadaverine yield after being reused 10 times at acidic and alkaline pH values than that of a recombinant E. coli strain overexpressing an inducible lysine decarboxylase (CadA), a conventional cadaverine producer (90 vs. 51% at pH 6 and 55 vs. 15% at pH 8). This study reveals that EcLdcC is a promising whole-cell biocatalyst for the bio-based production of cadaverine from industrial grade l-lysine in comparison to EcCadA. © 2018, Springer Science+Business Media, LLC, part of Springer Nature. | * |
dc.description.sponsorship | Ministry of Trade, Industry and Energy | * |
dc.language | English | * |
dc.publisher | Humana Press Inc. | * |
dc.subject | Cadaverine | * |
dc.subject | l-Lysine | * |
dc.subject | Lysine decarboxylase | * |
dc.subject | Reusability | * |
dc.subject | Whole-cell biotransformation | * |
dc.title | Characterization of a Whole-Cell Biotransformation Using a Constitutive Lysine Decarboxylase from Escherichia coli for the High-Level Production of Cadaverine from Industrial Grade l-Lysine | * |
dc.type | Article | * |
dc.relation.issue | 4 | * |
dc.relation.volume | 185 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 909 | * |
dc.relation.lastpage | 924 | * |
dc.relation.journaltitle | Applied Biochemistry and Biotechnology | * |
dc.identifier.doi | 10.1007/s12010-018-2696-4 | * |
dc.identifier.wosid | WOS:000439974900004 | * |
dc.identifier.scopusid | 2-s2.0-85040972424 | * |
dc.author.google | Shin J. | * |
dc.author.google | Joo J.C. | * |
dc.author.google | Lee E. | * |
dc.author.google | Hyun S.M. | * |
dc.author.google | Kim H.J. | * |
dc.author.google | Park S.J. | * |
dc.author.google | Yang Y.-H. | * |
dc.author.google | Park K. | * |
dc.contributor.scopusid | 박시재(57191670770) | * |
dc.date.modifydate | 20240322131024 | * |