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A colorimetric and fluorescent probe for rapid detection of glutathione and its application to tissue specific bio-imaging in living cells and zebrafish

Title
A colorimetric and fluorescent probe for rapid detection of glutathione and its application to tissue specific bio-imaging in living cells and zebrafish
Authors
Chen L.Park J.-S.Wu D.Kim C.-H.Yoon J.
Ewha Authors
윤주영
SCOPUS Author ID
윤주영scopus
Issue Date
2018
Journal Title
Sensors and Actuators, B: Chemical
ISSN
0925-4005JCR Link
Citation
Sensors and Actuators, B: Chemical vol. 262, pp. 306 - 312
Keywords
GlutathioneLiving cellsOlfactory pitRhodamineTissue specificZebrafish
Publisher
Elsevier B.V.
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Owing to the many physiological functions of glutathione, fluorescent sensing systems that selectively and sensitively detect this biothiol in biological systems are in great demand. In this study, we developed a rhodamine-based probe to detect glutathione. The probe, which contains an α-bromoamide reactive center, responds rapidly and highly selectively to glutathione in both a colorimetric and “off/on” fluorescence manner. Upon addition of glutathione to a solution of the probe, up to an 80-fold enhancement in the intensity of fluorescence at 579 nm takes place, which can be visually observed by using a hand-held UV lamp (365 nm). Moreover, following addition of glutathione to the solution of the probe undergoes a color change from colorless to pink in association with the development of an absorption band at 560 nm. Consequently, glutathione detection can be made using “naked-eyes”. The results of studies aimed at exploring the cell permeability and intracellular GSH detection ability of the probe show that the probe can be utilized to sense endogenous and exogenous glutathione in HeLa cells. Moreover, the probe can be utilized to interrogate the oxidation states of glutathione, which are crucial to the maintenance of intracellular redox balances. Either addition of hydrogen peroxide or a lipopolysaccharide to solutions containing the probe and glutathione results in a remarkable decrease in the intensity of fluorescence due to their metabolism is in conjunction with glutathione oxidation. More interestingly, the probe can be used to detect glutathione in zebrafish with a high specificity for olfactory pit tissue. © 2018 Elsevier B.V.
DOI
10.1016/j.snb.2018.02.023
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자연과학대학 > 화학·나노과학전공 > Journal papers
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