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A rhodamine-based fluorescent probe for the detection of lysosomal pH changes in living cells
- A rhodamine-based fluorescent probe for the detection of lysosomal pH changes in living cells
- Lee D.; Swamy K.M.K.; Hong J.; Lee S.; Yoon J.
- Ewha Authors
- 윤주영; K.M.K Swamy
- SCOPUS Author ID
- Issue Date
- Journal Title
- Sensors and Actuators, B: Chemical
- Sensors and Actuators, B: Chemical vol. 266, pp. 416 - 421
- Cell imaging; Fluorescence; Lysosome; pH sensor; Rhodamine
- Elsevier B.V.
- SCI; SCIE; SCOPUS
- Document Type
- Sensing and monitoring of pH changes in live cells are of great importance for understanding of cellular signaling responses. In this study, we developed a highly sensitive and selective pH sensor, rhodamine-based fluorescence dye 1. The fluorescent emission of 1 at 588 nm increased with the pH decreasing (from pH 7.0–4.0) due to the ring-opening of spirolactam by H+. 1 exhibited good water solubility, high quantum yield, and fast response to pH changes. Moreover, 1 can stain lysosomes in living cells with high photostability and low cytotoxicity. Importantly, 1 was used for monitoring pH changes in lysosomes induced by chloroquine, artesunate and dexamethasone. Therefore, our work provides the potential of probe 1 for visualizing the intracellular changes of pH in clinical and medical applications. © 2018 Elsevier B.V.
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