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A rhodamine-based fluorescent probe for the detection of lysosomal pH changes in living cells

Title
A rhodamine-based fluorescent probe for the detection of lysosomal pH changes in living cells
Authors
Lee D.Swamy K.M.K.Hong J.Lee S.Yoon J.
Ewha Authors
윤주영K.M.K Swamy
SCOPUS Author ID
윤주영scopus
Issue Date
2018
Journal Title
Sensors and Actuators, B: Chemical
ISSN
0925-4005JCR Link
Citation
Sensors and Actuators, B: Chemical vol. 266, pp. 416 - 421
Keywords
Cell imagingFluorescenceLysosomepH sensorRhodamine
Publisher
Elsevier B.V.
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Sensing and monitoring of pH changes in live cells are of great importance for understanding of cellular signaling responses. In this study, we developed a highly sensitive and selective pH sensor, rhodamine-based fluorescence dye 1. The fluorescent emission of 1 at 588 nm increased with the pH decreasing (from pH 7.0–4.0) due to the ring-opening of spirolactam by H+. 1 exhibited good water solubility, high quantum yield, and fast response to pH changes. Moreover, 1 can stain lysosomes in living cells with high photostability and low cytotoxicity. Importantly, 1 was used for monitoring pH changes in lysosomes induced by chloroquine, artesunate and dexamethasone. Therefore, our work provides the potential of probe 1 for visualizing the intracellular changes of pH in clinical and medical applications. © 2018 Elsevier B.V.
DOI
10.1016/j.snb.2018.03.133
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
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