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Silencing of peroxiredoxin II by promoter methylation is necessary for the survival and migration of gastric cancer cells

Title
Silencing of peroxiredoxin II by promoter methylation is necessary for the survival and migration of gastric cancer cells
Authors
Hong S.H.Min C.Jun Y.Lee D.J.Kim S.H.Park J.H.Cheong J.H.Park Y.J.Kim S.-Y.Lee S.Kang S.W.
Ewha Authors
이상혁강상원박윤정
SCOPUS Author ID
이상혁scopus; 강상원scopus; 박윤정scopus
Issue Date
2018
Journal Title
Experimental & molecular medicine
ISSN
2092-6413JCR Link
Citation
Experimental & molecular medicine vol. 50, no. 2, pp. e443
Publisher
NLM (Medline)
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Document Type
Article
Abstract
Peroxiredoxin (Prx), a family of ubiquitous thiol peroxidases, functions as a redox signaling regulator that controls cellular H2O2 in mammalian cells and has recently received attention for being overexpressed in various cancer types. In this study, we show that Prx type II (PrxII) is rather silenced in gastric cancer cells. PrxII expression is severely downregulated in 9 out of the 28 gastric cancer cell lines. Strikingly, PrxII expression is completely lost in three cell lines, MKN28, MKN74 and SNU484. Loss of PrxII expression is due to DNA methyltransferase 1-dependent methylation at the promoter region of the PrxII gene. Restoration of PrxII expression using a retroviral system markedly reduces the colony-forming ability and migratory activity of both MKN28 and SNU484 cells by inhibiting Src kinase. Mechanistically, PrxII peroxidase activity is essential for regulating gastric cancer cell migration. Bioinformatics analysis from The Cancer Genome Atlas stomach cancer data (STAD) revealed significantly low PrxII expression in gastric cancer patients and a negative correlation between PrxII expression and methylation levels. More importantly, low PrxII expression also strongly correlates with poor survival in cancer patients. Thus our study suggests that PrxII may be the first thiol peroxidase that simultaneously regulates both survival and metastasis in gastric cancer cells with high clinical relevance.
DOI
10.1038/emm.2017.267
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자연과학대학 > 생명과학전공 > Journal papers
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