View : 4 Download: 0

Interaction between IgE-dependent histamine-releasing factor and triosephosphate isomerase in HeLa cells

Title
Interaction between IgE-dependent histamine-releasing factor and triosephosphate isomerase in HeLa cells
Authors
Moon J.-A.Kim H.-J.Lee K.
Ewha Authors
김화정이경림
SCOPUS Author ID
김화정scopus; 이경림scopus
Issue Date
2005
Journal Title
Korean Journal of Microbiology and Biotechnology
ISSN
1598-642XJCR Link
Citation
vol. 33, no. 4, pp. 255 - 259
Indexed
SCOPUS; KCI scopus
Abstract
IgE-dependent histamine-releasing factor (HRF) is found extracellularly to regulate the degranulation process of histamine in mast cells and basophils and known to play a predominant role in the pathogenesis of chronic allergic disease. HRF has been also identified in the intracellular region of the cell. Previously, we reported that HRF interacts with the 3rd cytoplasmic domain of the alpha subunit of Na,K-ATPase. To understand the molecular mechanism of the regulation of Na,K-ATPase activity by HRF, we investigated the interaction between HRF and TPI since TPI was obtained as HRF-interacting protein in HeLa cDNA library, using yeast two hybrid screening. Domain mapping study of the interaction between HRF and TPI revealed that the C-terminal region of the residue 156-249 of TPI is involved in the interaction with HRF. The interaction between HRF and TPI was confirmed by immunoprecipitation from HeLa cell extracts. Our results suggest that TPI is a HRF-binding protein and the interaction between HRF and TPI may thus affect Na,K-ATPase activity.
Appears in Collections:
약학대학 > 약학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE