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dc.contributor.author전길자-
dc.contributor.author이민영-
dc.date.accessioned2018-06-02T08:15:06Z-
dc.date.available2018-06-02T08:15:06Z-
dc.date.issued1997-
dc.identifier.issn1225-8687-
dc.identifier.otherOAK-17048-
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/244396-
dc.description.abstractInteractions between ganglioside GM3 and glucose transporter, GLUT1 were studied by measuring the effect of GM3 on steady-state and time-resolved fluorescence of purified GLUT1 in synthetic lipids and on the 3-O-methylglucose uptake by human erythrocytes. The intrinsic tryptophan fluorescence showed a GLUT 1 emission maximum of 335 nm, and increased in the presence of GM3 by 12% without shifting the emission maximum. The fluorescence lifetimes of intrinsic tryptophan on GLUT1 consisted of a long component of 7.8 ns and a short component of 2.3 ns and GM3 increased both lifetime components. Lifetime components were quenched by acrylamide and KI. Acrylamide-induced quenching of long-lifetime components was partly recovered by GM3. However, KI-induced quenching of short- and long-lifetime components was not rescued by GM3. The anisotropy of 1,6-diphenyl-1,3,5-hexatriene (DPH)-probed dimyristoylphosphatidylcholine (DMPC) model membrane was also increased with GM3 incorporation. The transport rate of 3-O-methylglucose increased by 20% with GM3 incorporation on the erythrocytes. Therefore, GM3 altered the environment of lipid membrane and induced the conformational change of GLUT1.-
dc.languageEnglish-
dc.titleEffect of ganglioside GM3 on the erythrocyte glucose transporter (GLUT1): Conformational changes measured by steady-state and time-resolved fluorescence spectroscopy-
dc.typeArticle-
dc.relation.issue4-
dc.relation.volume30-
dc.relation.indexSCOPUS-
dc.relation.startpage240-
dc.relation.lastpage245-
dc.relation.journaltitleJournal of Biochemistry and Molecular Biology-
dc.identifier.scopusid2-s2.0-0031500621-
dc.author.googleYoon H.-
dc.author.googleLee M.-
dc.author.googleJhon G.-J.-
dc.contributor.scopusid전길자(6701488476)-
dc.contributor.scopusid이민영(55582235800)-
dc.date.modifydate20190901081003-
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자연과학대학 > 화학·나노과학전공 > Journal papers
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