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Isolation of 5′-untranslational region of trout Cyp1A1 gene

Title
Isolation of 5′-untranslational region of trout Cyp1A1 gene
Authors
Roh Y.N.Sheen Y.Y.
Ewha Authors
신윤용
SCOPUS Author ID
신윤용scopus
Issue Date
1996
Journal Title
Archives of Pharmacal Research
ISSN
0253-6269JCR Link
Citation
Archives of Pharmacal Research vol. 19, no. 6, pp. 450 - 455
Indexed
SCIE; SCOPUS; KCI scopus
Document Type
Article
Abstract
The genomic DNA was prepared from trout liver which was treated with 3-methycholanthrene, and cloned into lambda EMBL3 at BamHI site. The genomic library was constructed via infections of these recombinant phages into E. coli K802, and screened by the most 5′-portion of trout CYP1A1 cDNA. After the screening of 10 9 clones of the amplified library, 12 positive clones were isolated, and subjected to further screenings. The results of southern blot hybridization of genomic DNA prepared from the positive clone showed the presence of a single gene of CYP1A1, and 3.5 Kb PstI fragment that hybridizes with the most 5′-region DNA of CYP1A1 cDNA. The restriction map of PstI fragment was determined by the restriction digestion with various enzymes. The nucleotide sequence of the upstream genomic DNA of CYP1A1 was determined by DNA sequencing of exonuclease III unidirectionally deleted PstI fragment DNA using [ 35S]dATP. This paper presented the upstream genomic DNA of CYP1A1 contained a part of coding region which was about 351 base pairs (from ATG to PstI site at 3563).
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약학대학 > 약학과 > Journal papers
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