Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 오억수 | - |
dc.date.accessioned | 2018-05-30T08:14:26Z | - |
dc.date.available | 2018-05-30T08:14:26Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0008-5472 | - |
dc.identifier.other | OAK-2983 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/243642 | - |
dc.description.abstract | Expression of syndecan-2, a transmembrane heparan sulfate proteoglycan, is crucial for the tumorigenic activity in colon carcinoma cells. However, despite the high-level expression of syndecan-2 in mesenchymal cells, few studies have addressed the function of syndecan-2 in sarcoma cells. In HT1080 fibrosarcoma cells, we found that syndecan-2 regulated migration, invasion into Matrigel, and anchorage-independent growth but not cell-extracellular matrix adhesion or proliferation, suggesting that syndecan-2 plays different functional roles in fibrosarcoma and colon carcinoma cells. Consistent with the increased cell migration/invasion of syndecan-2-overexpressing HT1080 cells, syndecan-2 overexpression increased phosphorylation and interaction of focal adhesion kinase (FAK) and phosphatidylinositol 3-kinase (PI3K), membrane localization of T-lymphoma invasion and metastasis gene-1 (Tiam-1), and activation of Rac. Syndecan-2-mediated cell migration/invasion of HT1080 cells was diminished when (a) cells were cotransfected with nonphosphorylatable mutant FAK Y397F or with other FAK mutants lacking PI3K interactions, (b) cells were treated with a specific PI3K inhibitor, or (c) levels of Tiam-1 were knocked down with small interfering RNAs. Furthermore, expression of several FAK mutants inhibited syndecan-2-mediated enhancement of anchorage-independent growth in HT1080 cells. Taken together, these data suggest that syndecan-2 regulates the tumorigenic activities of HT1080 fibrosarcoma cells and that FAK is a key regulator of syndecan-2-mediated tumorigenic activities. ©2005 American Association for Cancer Research. | - |
dc.language | English | - |
dc.title | Focal adhesion kinase regulates syndecan-2-mediated tumorigenic activity of HT1080 fibrosarcoma cells | - |
dc.type | Article | - |
dc.relation.issue | 21 | - |
dc.relation.volume | 65 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 9899 | - |
dc.relation.lastpage | 9905 | - |
dc.relation.journaltitle | Cancer Research | - |
dc.identifier.doi | 10.1158/0008-5472.CAN-05-1386 | - |
dc.identifier.wosid | WOS:000232967000040 | - |
dc.identifier.scopusid | 2-s2.0-27544449024 | - |
dc.author.google | Park H. | - |
dc.author.google | Han I. | - |
dc.author.google | Kwon H.J. | - |
dc.author.google | Oh E.-S. | - |
dc.contributor.scopusid | 오억수(7101967153) | - |
dc.date.modifydate | 20190901081003 | - |