Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 김원기 | - |
dc.date.accessioned | 2018-05-30T08:14:20Z | - |
dc.date.available | 2018-05-30T08:14:20Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 1018-2438 | - |
dc.identifier.other | OAK-3049 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/243606 | - |
dc.description.abstract | Background: Tissue-invading helminth parasites secrete a large amount of cysteine proteases that may play critical roles in tissue invasion and immune modulation. However, roles of excretory-secretory products (ESP) secreted by Paragonimus westermani in the activation and death of microglial cells in brain are poorly understood. Objectives: In the present study, we investigated whether ESP could regulate microglial nitric oxide (NO) production and viability. Methods: The NO production and cell viability were assessed by respectively measuring the formation of nitrite and the release of lactate dehyrogenase. Results: At a low (0.2 μg/ml) concentration, ESP significantly stimulated NO production with no apparent cell injury or death in cultured microglial cells. However, at high (≥2 μg/ml) concentrations, ESP induced severe cell death. Inhibition of inducible NO synthase significantly reduced the NO productivity, but not cytotoxicity, of ESP. Similarly, inhibitors of the extracellular signal-regulated kinase, p38 and nuclear factor kappa B also blocked only the NO productivity of ESP. Interestingly, heat inactivation did not hamper the ability of ESP to stimulate microglial NO production. Similarly, pretreatment with thiol-crosslinking reagents dramatically reduced both proteolytic activity and cytotoxicity of ESP, but did not alter NO production in microglial cells. Interestingly, although cysteine protease competitive inhibitors and thiol-alkylating reagents markedly reduced the proteolytic activity of ESP, they did not influence the NO productivity and cytotoxicity of ESP. Conclusion: The present results indicate that the NO production and cytotoxicity by ESP may be differentially regulated via unknown mechanisms, not related with cysteine protease activity. Copyright © 2006 S. Karger AG. | - |
dc.language | English | - |
dc.title | Excretory-secretory products produced by Paragonimus westermani differentially regulate the nitric oxide production and viability of microglial cells | - |
dc.type | Article | - |
dc.relation.issue | 1 | - |
dc.relation.volume | 139 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 16 | - |
dc.relation.lastpage | 24 | - |
dc.relation.journaltitle | International Archives of Allergy and Immunology | - |
dc.identifier.doi | 10.1159/000089518 | - |
dc.identifier.wosid | WOS:000233773600003 | - |
dc.identifier.scopusid | 2-s2.0-30044436968 | - |
dc.author.google | Jin Y. | - |
dc.author.google | Lee J.-C. | - |
dc.author.google | In Y.C. | - |
dc.author.google | Kim E.A. | - |
dc.author.google | Myeong H.S. | - |
dc.author.google | Kim W.-K. | - |
dc.contributor.scopusid | 김원기(34770946200) | - |
dc.date.modifydate | 20211210152058 | - |