Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 김원기 | - |
dc.date.accessioned | 2018-05-30T08:14:20Z | - |
dc.date.available | 2018-05-30T08:14:20Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.other | OAK-3054 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/243605 | - |
dc.description.abstract | Mitogen-activated protein (MAP) kinase phosphatase-1 (MKP-1) is a dual specificity phosphatase that negatively regulates the MAP kinases. In this study, we found that levels of MKP-1 expression were transiently decreased within 3 h, followed by an increase 6-9 h after H 2O 2-induced oxidative stress in human neuroblastoma SH-SY5Y cells. There was a strong negative correlation between MKP-1 expression and ERK1/2 phosphorylation levels. Treatment of cells with a proteasomal inhibitor MG132 decreased the oxidative stress-induced degradation of MKP-1, resulting in dephosphorylation of ERK1/2. MG132 potentiated hydrogen peroxide-induced cell death, which was attenuated by a phosphatase inhibitor sodium orthovanadate. Suppression of MKP-1 expression by transfection with siRNA duplexes specific to MKP-1 transcript resulted in a decrease in oxidative stress-induced cell death. These data therefore suggest that MKP-1, a negative regulator of ERK1/2, plays a proapoptotic role in oxidative stress-induced cell death in a neuronal cell line. © 2005 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.title | MKP-1 contributes to oxidative stress-induced apoptosis via inactivation of ERK1/2 in SH-SY5Y cells | - |
dc.type | Article | - |
dc.relation.issue | 4 | - |
dc.relation.volume | 338 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 1732 | - |
dc.relation.lastpage | 1738 | - |
dc.relation.journaltitle | Biochemical and Biophysical Research Communications | - |
dc.identifier.doi | 10.1016/j.bbrc.2005.10.143 | - |
dc.identifier.wosid | WOS:000233815900010 | - |
dc.identifier.scopusid | 2-s2.0-27844477729 | - |
dc.author.google | Kim G.S. | - |
dc.author.google | Choi Y.K. | - |
dc.author.google | Song S.S. | - |
dc.author.google | Kim W.-K. | - |
dc.author.google | Han B.H. | - |
dc.contributor.scopusid | 김원기(34770946200) | - |
dc.date.modifydate | 20211210152058 | - |