Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 오억수 | - |
dc.contributor.author | 윤영대 | - |
dc.date.accessioned | 2018-05-30T08:14:18Z | - |
dc.date.available | 2018-05-30T08:14:18Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.other | OAK-3075 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/243590 | - |
dc.description.abstract | The syndecans are known to form homologous oligomers that may be important for their functions. We have therefore determined the role of oligomerization of syndecan-2 and syndecan-4. A series of glutathione S-transferase-syndecan-2 and syndecan-4 chimeric proteins showed that all syndecan constructs containing the transmembrane domain formed SDS-resistant dimers, but not those lacking it. SDS-resistant dimer formation was hardly seen in the syndecan chimeras where each transmembrane domain was substituted with that of platelet-derived growth factor receptor (PDGFR). Increased MAPK activity was detected in HEK293T cells transfected with syndecan/PDGFR chimeras in a syndecan transmembrane domain-dependent fashion. The chimera-induced MAPK activation was independent of both ligand and extracellular domain, implying that the transmembrane domain is sufficient to induce dimerization/oligomerization in vivo. Furthermore, the syndecan chimeras were defective in syndecan-4-mediated focal adhesion formation and protein kinase Cα activation or in syndecan-2-mediated cell migration. Taken together, these data suggest that the transmembrane domains are sufficient for inducing dimerization and that transmembrane domain-induced oligomerization is crucial for syndecan-2 and syndecan-4 functions. © 2005 by The American Society for Biochemistry and Molecular Biology, Inc. | - |
dc.language | English | - |
dc.title | Transmembrane domain-induced oligomerization is crucial for the functions of syndecan-2 and syndecan-4 | - |
dc.type | Article | - |
dc.relation.issue | 52 | - |
dc.relation.volume | 280 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 42573 | - |
dc.relation.lastpage | 42579 | - |
dc.relation.journaltitle | Journal of Biological Chemistry | - |
dc.identifier.doi | 10.1074/jbc.M509238200 | - |
dc.identifier.wosid | WOS:000234200800014 | - |
dc.identifier.scopusid | 2-s2.0-30044440012 | - |
dc.author.google | Choi S. | - |
dc.author.google | Lee E. | - |
dc.author.google | Kwon S. | - |
dc.author.google | Park H. | - |
dc.author.google | Yi J.Y. | - |
dc.author.google | Kim S. | - |
dc.author.google | Han I.-O. | - |
dc.author.google | Yun Y. | - |
dc.author.google | Oh E.-S. | - |
dc.contributor.scopusid | 오억수(7101967153) | - |
dc.contributor.scopusid | 윤영대(7201731033) | - |
dc.date.modifydate | 20200901081003 | - |