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Regulation of castasterone level in primary roots of maize, Zea mays
- Title
- Regulation of castasterone level in primary roots of maize, Zea mays
- Authors
- Kim Y.-S.; Kim T.-W.; Chang S.C.; Pharis R.P.; Lee J.S.; Han T.-J.; Takatsuto S.; Cheong H.; Kim S.-K.
- Ewha Authors
- 이준승
- SCOPUS Author ID
- 이준승
- Issue Date
- 2006
- Journal Title
- Physiologia Plantarum
- ISSN
- 0031-9317
- Citation
- Physiologia Plantarum vol. 127, no. 1, pp. 28 - 37
- Indexed
- SCI; SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Gas chromatography-mass spectrometry analysis revealed that primary roots of maize contain 28-norcastasterone (28-norCS) and its biosynthetic precursors, cholesterol, and cholestanol, which suggests that the C27-brassinosteroid (C27-BR) biosynthetic pathway to generate 28-norCS is operative in the roots. A cell-free enzyme solution prepared from maize roots successfully mediated C24-methylation of 28-norCS to produce castasterone (CS) with the aid of S-adenosyl-l-methionine, which indicates that CS can be generated through C27-BR biosynthesis, as well as C28-BR biosynthesis, in maize roots. Enzymatic conversion study using the cell-free enzyme solution demonstrated that CS is converted into 26-norCS in the enzyme solution. Exogenously applied 28-norCS and 26-norCS showed less activity than CS in the activation of gravitropic curvature and inhibition of root elongation. Taken together, a steady-state level of CS, the active BR in maize roots, seems to be strictly controlled by complicated processes such as C28- and C27-BR biosynthesis and biodegradation by C26-demethylation to exert its biological activity. Copyright © Physiologia Plantarum 2006.
- DOI
- 10.1111/j.1399-3054.2006.00650.x
- Appears in Collections:
- 자연과학대학 > 생명과학전공 > Journal papers
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