View : 609 Download: 0
Biomimetic nanofibrous scaffolds: Preparation and characterization of chitin/silk fibroin blend nanofibers
- Title
- Biomimetic nanofibrous scaffolds: Preparation and characterization of chitin/silk fibroin blend nanofibers
- Authors
- Park K.E.; Jung S.Y.; Lee S.J.; Min B.-M.; Park W.H.
- Ewha Authors
- 이승진
- SCOPUS Author ID
- 이승진
- Issue Date
- 2006
- Journal Title
- International Journal of Biological Macromolecules
- ISSN
- 0141-8130
- Citation
- International Journal of Biological Macromolecules vol. 38, no. 3-5, pp. 165 - 173
- Indexed
- SCI; SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Electrospinning of chitin/silk fibroin (SF) blend solutions in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) was investigated to fabricate a biomimetic nanostructured scaffolds for tissue engineering. The morphology of the electrospun chitin/SF blend nanofibers was investigated with a field emission scanning electron microscope (FE-SEM). The average diameters of chitin/SF blend fibers decreased from 920 to 340 nm, with the increase of chitin content in blend compositions. The miscibility of chitin/SF blend fibers was examined by solution viscosity measurement. The chitin and SF were immiscible in the as-spun nanofibrous structure. The dimensional stability of chitin/SF blend nanofibers, with or without water vapor after-treatment, was conducted by immersing in water. As-spun SF-rich blend nanofibrous matrices were lost their fibrous structure after the water immersion for 24 h, and then changed into membrane-like structure. On the contrary, nanofibrous structures of water vapor-treated SF-rich blends were almost maintained. To assay the cytocompatibility and cell behavior on the chitin/SF blend nanofibrous scaffolds, cell attachment and spreading of normal human epidermal keratinocyte and fibroblasts seeded on the scaffolds were studied. Our results indicate that chitin/SF blend nanofibrous matrix, particularly the one that contained 75% chitin and 25% SF, could be a potential candidate for tissue engineering scaffolds because it has both biomimetic three-dimensional structure and an excellent cell attachment and spreading for NHEK and NHEF. © 2006 Elsevier B.V. All rights reserved.
- DOI
- 10.1016/j.ijbiomac.2006.03.003
- Appears in Collections:
- 약학대학 > 약학과 > Journal papers
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML