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The major target of the endogenously generated reactive oxygen species in response to insulin stimulation is Phosphatase and Tensin homolog and not phosphoinositide-3 kinase (PI-3 kinase) in the PI-3 kinase/Akt pathway
- Title
- The major target of the endogenously generated reactive oxygen species in response to insulin stimulation is Phosphatase and Tensin homolog and not phosphoinositide-3 kinase (PI-3 kinase) in the PI-3 kinase/Akt pathway
- Authors
- Seo J.H.; Ahn Y.; Lee S.-R.; Yeo C.Y.; Hur K.C.
- Ewha Authors
- 허규정; 여창열
- SCOPUS Author ID
- 허규정; 여창열
- Issue Date
- 2005
- Journal Title
- Molecular Biology of the Cell
- ISSN
- 1059-1524
- Citation
- Molecular Biology of the Cell vol. 16, no. 1, pp. 348 - 357
- Indexed
- SCI; SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Phosphoinositide-3 kinase (PI-3 kinase) and its downstream signaling molecules PDK-1 and Akt were analyzed in SK-N-SH and SK-N-BE(2) human neuroblastoma cell lines. When cells were stimulated with insulin, PI-3 kinase was activated in both cell lines, whereas the translocation of PDK-1 to the membrane fraction and phosphorylated Akt were observed only in SK-N-SH cells. Analyses of the insulin-mediated reactive oxygen species (ROS) generation and Phosphatase and Tensin homolog (PTEN) oxidation indicate that PTEN oxidation occurred in SK-N-SH cells, which can produce ROS, but not in SK-N-BE(2) cells, which cannot increase ROS in response to insulin stimulation. When SK-N-SH cells were pretreated with the NADPH oxidase inhibitor diphenyleneiodonium chloride before insulin stimulation, insulin-mediated translocation of PDK-1 to the membrane fraction and phosphorylation of Akt were remarkably reduced, whereas PI-3 kinase activity was not changed significantly. These results indicate that not only PI-3 kinase activation but also inhibition of PTEN by ROS is needed to increase cellular level of phosphatidylinositol 3,4,5-trisphosphate for recruiting downstream signaling molecules such as PDK-1 and Akt in insulin-mediated signaling. Moreover, the ROS generated by insulin stimulation mainly contributes to the inactivation of PTEN and not to the activation of PI-3 kinase in the PI-3 kinase/Akt pathway.
- DOI
- 10.1091/mbc.E04-05-0369
- Appears in Collections:
- 일반대학원 > 바이오융합과학과 > Journal papers
- Files in This Item:
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