Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 허규정 | - |
dc.contributor.author | 여창열 | - |
dc.date.accessioned | 2018-05-18T08:15:23Z | - |
dc.date.available | 2018-05-18T08:15:23Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1059-1524 | - |
dc.identifier.other | OAK-2516 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/243246 | - |
dc.description.abstract | Phosphoinositide-3 kinase (PI-3 kinase) and its downstream signaling molecules PDK-1 and Akt were analyzed in SK-N-SH and SK-N-BE(2) human neuroblastoma cell lines. When cells were stimulated with insulin, PI-3 kinase was activated in both cell lines, whereas the translocation of PDK-1 to the membrane fraction and phosphorylated Akt were observed only in SK-N-SH cells. Analyses of the insulin-mediated reactive oxygen species (ROS) generation and Phosphatase and Tensin homolog (PTEN) oxidation indicate that PTEN oxidation occurred in SK-N-SH cells, which can produce ROS, but not in SK-N-BE(2) cells, which cannot increase ROS in response to insulin stimulation. When SK-N-SH cells were pretreated with the NADPH oxidase inhibitor diphenyleneiodonium chloride before insulin stimulation, insulin-mediated translocation of PDK-1 to the membrane fraction and phosphorylation of Akt were remarkably reduced, whereas PI-3 kinase activity was not changed significantly. These results indicate that not only PI-3 kinase activation but also inhibition of PTEN by ROS is needed to increase cellular level of phosphatidylinositol 3,4,5-trisphosphate for recruiting downstream signaling molecules such as PDK-1 and Akt in insulin-mediated signaling. Moreover, the ROS generated by insulin stimulation mainly contributes to the inactivation of PTEN and not to the activation of PI-3 kinase in the PI-3 kinase/Akt pathway. | - |
dc.language | English | - |
dc.title | The major target of the endogenously generated reactive oxygen species in response to insulin stimulation is Phosphatase and Tensin homolog and not phosphoinositide-3 kinase (PI-3 kinase) in the PI-3 kinase/Akt pathway | - |
dc.type | Article | - |
dc.relation.issue | 1 | - |
dc.relation.volume | 16 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 348 | - |
dc.relation.lastpage | 357 | - |
dc.relation.journaltitle | Molecular Biology of the Cell | - |
dc.identifier.doi | 10.1091/mbc.E04-05-0369 | - |
dc.identifier.wosid | WOS:000225954400035 | - |
dc.identifier.scopusid | 2-s2.0-11144279346 | - |
dc.author.google | Seo J.H. | - |
dc.author.google | Ahn Y. | - |
dc.author.google | Lee S.-R. | - |
dc.author.google | Yeo C.Y. | - |
dc.author.google | Hur K.C. | - |
dc.contributor.scopusid | 허규정(7005230301) | - |
dc.contributor.scopusid | 여창열(7102545519) | - |
dc.date.modifydate | 20230411114710 | - |