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dc.contributor.author허규정-
dc.contributor.author여창열-
dc.date.accessioned2018-05-18T08:15:23Z-
dc.date.available2018-05-18T08:15:23Z-
dc.date.issued2005-
dc.identifier.issn1059-1524-
dc.identifier.otherOAK-2516-
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/243246-
dc.description.abstractPhosphoinositide-3 kinase (PI-3 kinase) and its downstream signaling molecules PDK-1 and Akt were analyzed in SK-N-SH and SK-N-BE(2) human neuroblastoma cell lines. When cells were stimulated with insulin, PI-3 kinase was activated in both cell lines, whereas the translocation of PDK-1 to the membrane fraction and phosphorylated Akt were observed only in SK-N-SH cells. Analyses of the insulin-mediated reactive oxygen species (ROS) generation and Phosphatase and Tensin homolog (PTEN) oxidation indicate that PTEN oxidation occurred in SK-N-SH cells, which can produce ROS, but not in SK-N-BE(2) cells, which cannot increase ROS in response to insulin stimulation. When SK-N-SH cells were pretreated with the NADPH oxidase inhibitor diphenyleneiodonium chloride before insulin stimulation, insulin-mediated translocation of PDK-1 to the membrane fraction and phosphorylation of Akt were remarkably reduced, whereas PI-3 kinase activity was not changed significantly. These results indicate that not only PI-3 kinase activation but also inhibition of PTEN by ROS is needed to increase cellular level of phosphatidylinositol 3,4,5-trisphosphate for recruiting downstream signaling molecules such as PDK-1 and Akt in insulin-mediated signaling. Moreover, the ROS generated by insulin stimulation mainly contributes to the inactivation of PTEN and not to the activation of PI-3 kinase in the PI-3 kinase/Akt pathway.-
dc.languageEnglish-
dc.titleThe major target of the endogenously generated reactive oxygen species in response to insulin stimulation is Phosphatase and Tensin homolog and not phosphoinositide-3 kinase (PI-3 kinase) in the PI-3 kinase/Akt pathway-
dc.typeArticle-
dc.relation.issue1-
dc.relation.volume16-
dc.relation.indexSCI-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage348-
dc.relation.lastpage357-
dc.relation.journaltitleMolecular Biology of the Cell-
dc.identifier.doi10.1091/mbc.E04-05-0369-
dc.identifier.wosidWOS:000225954400035-
dc.identifier.scopusid2-s2.0-11144279346-
dc.author.googleSeo J.H.-
dc.author.googleAhn Y.-
dc.author.googleLee S.-R.-
dc.author.googleYeo C.Y.-
dc.author.googleHur K.C.-
dc.contributor.scopusid허규정(7005230301)-
dc.contributor.scopusid여창열(7102545519)-
dc.date.modifydate20190301081000-


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