View : 147 Download: 0

A novel adenosine analog, thio-Cl-IB-MECA, induces G0/G 1 cell cycle arrest and apoptosis in human promyelocytic leukemia HL-60 cells

Title
A novel adenosine analog, thio-Cl-IB-MECA, induces G0/G 1 cell cycle arrest and apoptosis in human promyelocytic leukemia HL-60 cells
Authors
Lee E.-J.Min H.-Y.Chung H.-J.Park E.-J.Shin D.-H.Lak S.J.Sang K.L.
Ewha Authors
정낙신이상국
Issue Date
2005
Journal Title
Biochemical Pharmacology
ISSN
0006-2952JCR Link
Citation
Biochemical Pharmacology vol. 70, no. 6, pp. 918 - 924
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Human A3 adenosine receptor (A3AR) agonists have been shown to play important roles in several physiological and pathological processes, including growth inhibition of human cancer cells. On this line, we recently found that a novel adenosine analog, 2-chloro-N6-(3- iodobenzyl)-4′-thioadenosine-5′-N-methyluronamide (thio-Cl-IB-MECA) was a potent human A3AR agonist, and is superior to a known agonist Cl-IB-MECA [Jeong LS, Jin DZ, Kim HO, Shin DH, Moon HR, Gunaga P, et al. J Med Chem 2003;46:3775]. Here, we report that a novel A3AR agonist, thio-Cl-IB-MECA inhibited the growth of human promyelocytic leukemia HL-60 cells by arresting cell cycle and induction of apoptosis. Thio-Cl-IB-MECA induced the cell cycle arrest of G0/G1 in the early time and at lower concentration (up to 25 μM). At higher concentration (50 μM), the apoptotic cell deaths were manifested by observation of the increase of sub-G0 phase of cell cycle distribution, DNA fragmentation and poly(ADP-ribose) polymerase (PARP) cleavage. In addition, the down-regulation of checkpoint protein cyclin D1 and c-myc by thio-Cl-IB-MECA was well correlated with the arrest of cell cycle transition of G1 to S phase. Further study revealed that the growth inhibitory activity of thio-Cl-IB-MECA is also related with the modulation of Wnt signaling pathway. The levels of β-catenin, phosphorylated forms of GSK-β and Akt were down-regulated by the treatment of thio-Cl-IB-MECA (10 nM) in a time-dependent manner, providing one of plausible mechanistic evidence for the involvement of the Wnt signaling pathway in the HL-60 cell growth inhibitory effects by thio-Cl-IB-MECA. These results suggest that a novel A3AR agonist, thio-Cl-IB-MECA can down-regulate Wnt signaling, inhibit proliferation and induce apoptosis in HL-60 leukemia cells, and thus provide the possibility of this compound in the potential therapeutic value of the treatment of leukemia. © 2005 Elsevier Inc. All rights reserved.
DOI
10.1016/j.bcp.2005.06.017
Appears in Collections:
약학대학 > 약학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE