Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 최철희 | - |
dc.date.accessioned | 2018-05-02T08:15:38Z | - |
dc.date.available | 2018-05-02T08:15:38Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 0894-1491 | - |
dc.identifier.other | OAK-2214 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/242755 | - |
dc.description.abstract | We have previously demonstrated that chloroquine may evoke inflammatory responses in the central nervous system by inducing expression of pro-inflammatory cytokines by astroglial cells. In this study, we further examined the molecular mechanism responsible for chloroquine-induced activation of NF-κB and subsequent expression of chemokines by astroglial cells. We observed that (1) chloroquine induced expression of chemokines such as CCL2 and CXCL8 in a dose- and time-dependent manner in human astroglial cells; (2) other lysosomotropic agents such as ammonium chloride and bafilomycin A 1 had minimal effects on chemokine expression; (3) inhibition of NF-κB by MG-132 and TPCK suppressed chloroquine-induced mRNA expression of chemokines; (4) chloroquine increased the intracellular level of reactive oxygen species (ROS) in a dose- and time-dependent manner by human astroglial cells, but not by monocytic/microglial cells; (5) chloroquine-induced increase of intracellular ROS level was suppressed by pre-incubation with diphenyl iodonium (DPI) and N-acetyl cysteine (NAC); and (6) inhibition of chloroquine-induced ROS production by DPI or NAC suppressed chloroquine-mediated activation of NF-κB and subsequent mRNA expression of chemokines in astroglial cells. These results collectively suggest that chloroquine generates ROS, which is responsible for NF-κB activation and subsequent expression of pro-inflammatory chemokines in human astroglial cells. © 2004 Wiley-Liss, Inc. | - |
dc.language | English | - |
dc.title | Reactive oxygen species mediate chloroquine-induced expression of chemokines by human astroglial cells | - |
dc.type | Article | - |
dc.relation.issue | 1 | - |
dc.relation.volume | 47 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 9 | - |
dc.relation.lastpage | 20 | - |
dc.relation.journaltitle | GLIA | - |
dc.identifier.doi | 10.1002/glia.20017 | - |
dc.identifier.wosid | WOS:000222015400002 | - |
dc.identifier.scopusid | 2-s2.0-3042837762 | - |
dc.author.google | Pare J. | - |
dc.author.google | Choi K. | - |
dc.author.google | Jeong E. | - |
dc.author.google | Kwon D. | - |
dc.author.google | Benveniste E.N. | - |
dc.author.google | Choi C. | - |
dc.contributor.scopusid | 최철희(55574827700) | - |
dc.date.modifydate | 20211210152234 | - |