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Effect of apoptosis-associated speck-like protein containing a caspase recruitment domain on vaccine efficacy: Overcoming the effects of its deficiency with aluminum hydroxide adjuvant

Title
Effect of apoptosis-associated speck-like protein containing a caspase recruitment domain on vaccine efficacy: Overcoming the effects of its deficiency with aluminum hydroxide adjuvant
Authors
Lee, Deuk-KiLee, Eun-YoungKim, Ryoon-HoKwak, Hye-WonKim, Joo YoungKim, HunKang, Kyung-WonLee, Sang-MyeongPark, Jong-HwanChang, JunNam, Jae-Hwan
Ewha Authors
장준김주영
SCOPUS Author ID
장준scopus; 김주영scopusscopus
Issue Date
2018
Journal Title
MICROBIOLOGY AND IMMUNOLOGY
ISSN
0385-5600JCR Link

1348-0421JCR Link
Citation
MICROBIOLOGY AND IMMUNOLOGY vol. 62, no. 3, pp. 176 - 186
Keywords
alumASCHPV vaccineinfluenza vaccine
Publisher
WILEY
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Host factors such as nutritional status and immune cell state are important for vaccine efficacy. Inflammasome activation may be important for triggering vaccine-induced humoral and cell-mediated immune responses. Formulations with alum as a typical adjuvant to overcome the effects of host factors have recently been shown to induce inflammasome activation, which augments vaccine efficacy. Apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) is one of the main components of inflammasomes, but it is not clear whether ASC affects the vaccine-induced immune response. Herein, we used two types of vaccines: inactivated influenza vaccine not formulated with alum, and HPV vaccine formulated with alum. We gave the vaccines to ASC knockout (ASC(-/-)) mice to investigate the role of ASC in vaccine efficacy. Influenza vaccine-immunized ASC(-/-) mice did not show antibody titers in week 2 after the first vaccination. After boosting, the antibody titer in ASC(-/-) mice was about half that in wild type (WT) mice. Furthermore, a cytotoxic T-lymphocyte response against influenza vaccine was not induced in ASC(-/-) mice. Therefore, vaccinated ASC(-/-) mice did not show effective protection against viral challenge. ASC(-/-) mice immunized with alum-formulated HPV vaccine showed similar antibody titers and T-cell proliferation compared with immunized WT mice. However, the HPV vaccine without alum induced up to threefold lower titers of HPV-specific antibody titers in ASC(-/-) mice compared with those in WT mice. These findings suggest that alum in vaccine can overcome the ASC-deficient condition.
DOI
10.1111/1348-0421.12569
Appears in Collections:
약학대학 > 약학과 > Journal papers
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