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Effect of apoptosis-associated speck-like protein containing a caspase recruitment domain on vaccine efficacy: Overcoming the effects of its deficiency with aluminum hydroxide adjuvant
- Title
- Effect of apoptosis-associated speck-like protein containing a caspase recruitment domain on vaccine efficacy: Overcoming the effects of its deficiency with aluminum hydroxide adjuvant
- Authors
- Lee, Deuk-Ki; Lee, Eun-Young; Kim, Ryoon-Ho; Kwak, Hye-Won; Kim, Joo Young; Kim, Hun; Kang, Kyung-Won; Lee, Sang-Myeong; Park, Jong-Hwan; Chang, Jun; Nam, Jae-Hwan
- Ewha Authors
- 장준; 김주영
- SCOPUS Author ID
- 장준; 김주영
- Issue Date
- 2018
- Journal Title
- MICROBIOLOGY AND IMMUNOLOGY
- ISSN
- 0385-5600
1348-0421
- Citation
- MICROBIOLOGY AND IMMUNOLOGY vol. 62, no. 3, pp. 176 - 186
- Keywords
- alum; ASC; HPV vaccine; influenza vaccine
- Publisher
- WILEY
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Host factors such as nutritional status and immune cell state are important for vaccine efficacy. Inflammasome activation may be important for triggering vaccine-induced humoral and cell-mediated immune responses. Formulations with alum as a typical adjuvant to overcome the effects of host factors have recently been shown to induce inflammasome activation, which augments vaccine efficacy. Apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) is one of the main components of inflammasomes, but it is not clear whether ASC affects the vaccine-induced immune response. Herein, we used two types of vaccines: inactivated influenza vaccine not formulated with alum, and HPV vaccine formulated with alum. We gave the vaccines to ASC knockout (ASC(-/-)) mice to investigate the role of ASC in vaccine efficacy. Influenza vaccine-immunized ASC(-/-) mice did not show antibody titers in week 2 after the first vaccination. After boosting, the antibody titer in ASC(-/-) mice was about half that in wild type (WT) mice. Furthermore, a cytotoxic T-lymphocyte response against influenza vaccine was not induced in ASC(-/-) mice. Therefore, vaccinated ASC(-/-) mice did not show effective protection against viral challenge. ASC(-/-) mice immunized with alum-formulated HPV vaccine showed similar antibody titers and T-cell proliferation compared with immunized WT mice. However, the HPV vaccine without alum induced up to threefold lower titers of HPV-specific antibody titers in ASC(-/-) mice compared with those in WT mice. These findings suggest that alum in vaccine can overcome the ASC-deficient condition.
- DOI
- 10.1111/1348-0421.12569
- Appears in Collections:
- 약학대학 > 약학과 > Journal papers
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