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Aberrant single-minded homolog 1 methylation as a potential biomarker for cervical cancer

Title
Aberrant single-minded homolog 1 methylation as a potential biomarker for cervical cancer
Authors
Kim H.-J.Kim C.Y.Jin J.Bae M.K.Kim Y.H.Ju W.Kim S.C.
Ewha Authors
김승철주웅김윤환
SCOPUS Author ID
김승철scopus; 주웅scopus; 김윤환scopus
Issue Date
2018
Journal Title
Diagnostic Cytopathology
ISSN
8755-1039JCR Link
Citation
Diagnostic Cytopathology vol. 46, no. 1, pp. 15 - 21
Keywords
cervical cancercirculating cell-free DNAdiagnostic biomarkerDNA methylationsingle-minded homolog 1(SIM1)
Publisher
John Wiley and Sons Inc.
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Background: The aim of this study is to evaluate the possibility of using the methylation status of single-minded homolog 1 (SIM1) as a diagnostic biomarker for cervical cancer. Methods: All the patient and normal specimens including the normal cervix (n = 10), cervical cancer tissues (n = 45), blood (n = 45), and cervical brush specimens (n = 110) were retrospectively obtained. Quantitative methylation-specific PCR was performed to detect SIM1 methylation in primary tumors, cervical brush specimens, and plasma circulating cell-free DNA (ccfDNA). SIM1 expression was detected by western blot analysis. Results: We found that SIM1 was highly methylated in the majority of the cervical cancer tissues that we tested, but not in any of the normal tissues. Hypermethylation of SIM1 led to a pronounced reduction in SIM1 expression in cervical cancer tissues compared with normal cervix. SIM1 methylation status on cervical brush specimens also distinguished cervical cancer from normal, cervical intraepithelial neoplasia (CIN) 1 and 2. The degree of SIM1 methylation was significantly associated with the severity of the disease (Ptrend <.0001). We also investigated the possibility of detecting methylated SIM1 in plasma ccfDNA from cervical cancer patients. Methylated SIM1 was detected in 36.6% (15/41) of ccfDNA samples, and concordance rate with the matched cancer tissues was 41.5% (17/41) with sensitivity 38.5% and specificity 100%. Conclusion: This study has shown that SIM1 is frequently hypermethylated in cervical cancer, compared with normal cervix tissue, CIN1 and 2 samples, suggesting that the methylation status of SIM1 could be a potential diagnostic biomarker for cervical cancer. © 2017 Wiley Periodicals, Inc.
DOI
10.1002/dc.23838
Appears in Collections:
의과대학 > 의학과 > Journal papers
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