Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 김승철 | * |
dc.contributor.author | 주웅 | * |
dc.contributor.author | 김윤환 | * |
dc.date.accessioned | 2018-02-27T16:30:36Z | - |
dc.date.available | 2018-02-27T16:30:36Z | - |
dc.date.issued | 2018 | * |
dc.identifier.issn | 8755-1039 | * |
dc.identifier.other | OAK-21796 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/240011 | - |
dc.description.abstract | Background: The aim of this study is to evaluate the possibility of using the methylation status of single-minded homolog 1 (SIM1) as a diagnostic biomarker for cervical cancer. Methods: All the patient and normal specimens including the normal cervix (n = 10), cervical cancer tissues (n = 45), blood (n = 45), and cervical brush specimens (n = 110) were retrospectively obtained. Quantitative methylation-specific PCR was performed to detect SIM1 methylation in primary tumors, cervical brush specimens, and plasma circulating cell-free DNA (ccfDNA). SIM1 expression was detected by western blot analysis. Results: We found that SIM1 was highly methylated in the majority of the cervical cancer tissues that we tested, but not in any of the normal tissues. Hypermethylation of SIM1 led to a pronounced reduction in SIM1 expression in cervical cancer tissues compared with normal cervix. SIM1 methylation status on cervical brush specimens also distinguished cervical cancer from normal, cervical intraepithelial neoplasia (CIN) 1 and 2. The degree of SIM1 methylation was significantly associated with the severity of the disease (Ptrend <.0001). We also investigated the possibility of detecting methylated SIM1 in plasma ccfDNA from cervical cancer patients. Methylated SIM1 was detected in 36.6% (15/41) of ccfDNA samples, and concordance rate with the matched cancer tissues was 41.5% (17/41) with sensitivity 38.5% and specificity 100%. Conclusion: This study has shown that SIM1 is frequently hypermethylated in cervical cancer, compared with normal cervix tissue, CIN1 and 2 samples, suggesting that the methylation status of SIM1 could be a potential diagnostic biomarker for cervical cancer. © 2017 Wiley Periodicals, Inc. | * |
dc.language | English | * |
dc.publisher | John Wiley and Sons Inc. | * |
dc.subject | cervical cancer | * |
dc.subject | circulating cell-free DNA | * |
dc.subject | diagnostic biomarker | * |
dc.subject | DNA methylation | * |
dc.subject | single-minded homolog 1(SIM1) | * |
dc.title | Aberrant single-minded homolog 1 methylation as a potential biomarker for cervical cancer | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 46 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 15 | * |
dc.relation.lastpage | 21 | * |
dc.relation.journaltitle | Diagnostic Cytopathology | * |
dc.identifier.doi | 10.1002/dc.23838 | * |
dc.identifier.wosid | WOS:000417747800003 | * |
dc.identifier.scopusid | 2-s2.0-85037565582 | * |
dc.author.google | Kim H.-J. | * |
dc.author.google | Kim C.Y. | * |
dc.author.google | Jin J. | * |
dc.author.google | Bae M.K. | * |
dc.author.google | Kim Y.H. | * |
dc.author.google | Ju W. | * |
dc.author.google | Kim S.C. | * |
dc.contributor.scopusid | 김승철(35264000100) | * |
dc.contributor.scopusid | 주웅(8873659700) | * |
dc.contributor.scopusid | 김윤환(55763947200) | * |
dc.date.modifydate | 20240220115825 | * |