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Aptamer-incorporated DNA Holliday junction for the targeted delivery of siRNA

Title
Aptamer-incorporated DNA Holliday junction for the targeted delivery of siRNA
Authors
Jeong, Eun HyeJeong, HansaemJang, BoraKim, BoyoungKim, MinjeongKwon, HyokyoungLee, KyuriLee, Hyukjin
Ewha Authors
이혁진이규리
SCOPUS Author ID
이혁진scopus
Issue Date
2017
Journal Title
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
ISSN
1876-794XJCR Link1226-086XJCR Link
Citation
vol. 56, pp. 55 - 61
Keywords
AptamersiRNANucleic acid nanostructuresCarrier-free delivery system
Publisher
ELSEVIER SCIENCE INC
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
There have been unmet needs of developing carrier-free siRNA delivery systems to overcome the undesirable cellular toxicity and immunogenicity of conventional delivery systems such as cationic lipids and polymers. Various nucleic acid nanostructures have been proposed to achieve this goal, however many of them required potent ligands for the intracellular delivery of siRNA. Among various ligands, aptamers received much interest due to their facile preparation process as well as a high binding affinity toward target receptors. In addition, nucleic acid based aptamers can be easily incorporated with various nucleic acid nanostructures through a simple base-pair hybridization. In this study, aptamer incorporated siRNA (Apt-siRNA) was self-assembled with DNA Holliday junction for enhancing targeted delivery of siRNA to the Mucin 1 (MUC1) overexpressing cancer cells. Molecularly self-assembled Holliday DNA junction with Apt-siRNA was analyzed to confirm their cellular uptake and gene silencing as compared to the Apt-siRNA alone in the GFP expressing KB cells. The multivalent Apt-siRNA DNA nanostructure (Holliday-Apt-siRNA) clearly showed their superior potency over the Apt-siRNA alone suggesting their use in carrier-free siRNA delivery systems. (C) 2017 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
DOI
10.1016/j.jiec.2017.07.025
Appears in Collections:
약학대학 > 약학과 > Journal papers
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