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Layered Double Hydroxide and Polypeptide Thermogel Nanocomposite System for Chondrogenic Differentiation of Stem Cells

Title
Layered Double Hydroxide and Polypeptide Thermogel Nanocomposite System for Chondrogenic Differentiation of Stem Cells
Authors
Lee S.S.Choi G.E.Lee H.J.Kim Y.Choy J.-H.Jeong B.
Ewha Authors
정병문최진호최고은
SCOPUS Author ID
정병문scopus; 최진호scopus
Issue Date
2017
Journal Title
ACS Applied Materials and Interfaces
ISSN
1944-8244JCR Link
Citation
vol. 9, no. 49, pp. 42668 - 42675
Keywords
3D culturechondrogenic differentiationlayered double hydroxide (LDH)Solgel transitionstem cell
Publisher
American Chemical Society
Indexed
SCI; SCIE; SCOPUS scopus
Abstract
Stem cell therapy for damaged cartilage suffers from low rates of retention, survival, and differentiation into chondrocytes at the target site. To solve these problems, here we propose a two-dimensional/three-dimensional (2D/3D) nanocomposite system. As a new two-dimensional (2D) material, hexagonal layered double hydroxides (LDHs) with a uniform lateral length of 2-3 μm were prepared by a hydrothermal process. Then, tonsil-derived mesenchymal stem cells (TMSCs), arginylglycylaspartic acid-coated LDHs, and kartogenin (KGN) were incorporated into the gel through the thermal-energy-driven gelation of the system. The cells exhibited a tendency to aggregate in the nanocomposite system. In particular, chondrogenic biomarkers of type II collagen and transcription factor SOX 9 significantly increased at both the mRNA and protein levels in the nanocomposite system, compared to the pure thermogel systems. The inorganic 2D materials increased the rigidity of the matrix, slowed down the release of a soluble factor (KGN), and improved cell-material interactions in the gel. The current 2D/3D nanocomposite system of bioactive LDH/thermogel can be a new platform material overcoming drawbacks of hydrogel-based 3D cell culture systems and is eventually expected to be applied as an injectable stem cell therapy. © 2017 American Chemical Society.
DOI
10.1021/acsami.7b17173
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
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