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Exogenous Hydrogen Peroxide Induces Lipid Raft-Mediated STAT-6 Activation in T Cells

Title
Exogenous Hydrogen Peroxide Induces Lipid Raft-Mediated STAT-6 Activation in T Cells
Authors
Kim, Hee JaLim, JiwooJang, Young-SoonShin, Eui-CheolKim, Hyung-RanSeoh, Ju-YoungLee, Ji SooLee, Soon NamKang, Jihee LeeChoi, Youn-Hee
Ewha Authors
서주영이지희이순남이지수최윤희
SCOPUS Author ID
서주영scopus; 이지희scopus; 이순남scopus; 이지수scopus; 최윤희scopus
Issue Date
2017
Journal Title
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
ISSN
1015-8987JCR Link

1421-9778JCR Link
Citation
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY vol. 42, no. 6, pp. 2467 - 2480
Keywords
Reactive oxygen species (ROS)IL-4STAT-6Lipid raftsTh2 cells
Publisher
KARGER
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Background/Aims: CD4+ T cells are a critical component of the adaptive immune response. While the mechanisms controlling the differentiation of the Th1, Th17, and regulatory T cell subsets from naive CD4+ T cells are well described, the factors that induce Th2 differentiation are still largely unknown. Methods: The effects of treatment with exogenous H2O2 on STAT-6 phosphorylation and activation in T cells were examined by imnnunoblotting, imnnunofluorescence and gel shift assay. Anti-CD3 antibody and methyl-beta-cyclodextrin were utilized to induce lipid raft assembly and to investigate the involvement of lipid rafts, respectively. Results: Jurkat and EL-4T cells that were exposed to H2O2 showed rapid and strong STAT-6 phosphorylation, and the extent of STAT-6 phosphorylation was enhanced by co-treatment with anti-CD3 antibody. The effect of H2O2 on STAT-6 phosphorylation and translocation was inhibited by disruption of lipid rafts. STAT-6 activation in response to H2O2 treatment regulated IL-4 gene expression, and this response was strengthened by treatment with anti-CD3. Conclusion: Our results indicate that reactive oxygen species such as H2O2 can act on upstream and initiating factors for activation of STAT-6 in T cells and contribute to formation of a positive feedback loop between STAT-6 and IL-4 in the Th2 differentiation process. (C) 2017 The Author(s) Published by S. Karger AG, Basel
DOI
10.1159/000480210
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의과대학 > 의학과 > Journal papers
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