Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 서주영 | * |
dc.contributor.author | 이지희 | * |
dc.contributor.author | 이순남 | * |
dc.contributor.author | 이지수 | * |
dc.contributor.author | 최윤희 | * |
dc.date.accessioned | 2017-12-27T16:30:32Z | - |
dc.date.available | 2017-12-27T16:30:32Z | - |
dc.date.issued | 2017 | * |
dc.identifier.issn | 1015-8987 | * |
dc.identifier.issn | 1421-9778 | * |
dc.identifier.other | OAK-21577 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/239318 | - |
dc.description.abstract | Background/Aims: CD4+ T cells are a critical component of the adaptive immune response. While the mechanisms controlling the differentiation of the Th1, Th17, and regulatory T cell subsets from naive CD4+ T cells are well described, the factors that induce Th2 differentiation are still largely unknown. Methods: The effects of treatment with exogenous H2O2 on STAT-6 phosphorylation and activation in T cells were examined by imnnunoblotting, imnnunofluorescence and gel shift assay. Anti-CD3 antibody and methyl-beta-cyclodextrin were utilized to induce lipid raft assembly and to investigate the involvement of lipid rafts, respectively. Results: Jurkat and EL-4T cells that were exposed to H2O2 showed rapid and strong STAT-6 phosphorylation, and the extent of STAT-6 phosphorylation was enhanced by co-treatment with anti-CD3 antibody. The effect of H2O2 on STAT-6 phosphorylation and translocation was inhibited by disruption of lipid rafts. STAT-6 activation in response to H2O2 treatment regulated IL-4 gene expression, and this response was strengthened by treatment with anti-CD3. Conclusion: Our results indicate that reactive oxygen species such as H2O2 can act on upstream and initiating factors for activation of STAT-6 in T cells and contribute to formation of a positive feedback loop between STAT-6 and IL-4 in the Th2 differentiation process. (C) 2017 The Author(s) Published by S. Karger AG, Basel | * |
dc.language | English | * |
dc.publisher | KARGER | * |
dc.subject | Reactive oxygen species (ROS) | * |
dc.subject | IL-4 | * |
dc.subject | STAT-6 | * |
dc.subject | Lipid rafts | * |
dc.subject | Th2 cells | * |
dc.title | Exogenous Hydrogen Peroxide Induces Lipid Raft-Mediated STAT-6 Activation in T Cells | * |
dc.type | Article | * |
dc.relation.issue | 6 | * |
dc.relation.volume | 42 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 2467 | * |
dc.relation.lastpage | 2480 | * |
dc.relation.journaltitle | CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | * |
dc.identifier.doi | 10.1159/000480210 | * |
dc.identifier.wosid | WOS:000415239800027 | * |
dc.identifier.scopusid | 2-s2.0-85028448363 | * |
dc.author.google | Kim, Hee Ja | * |
dc.author.google | Lim, Jiwoo | * |
dc.author.google | Jang, Young-Soon | * |
dc.author.google | Shin, Eui-Cheol | * |
dc.author.google | Kim, Hyung-Ran | * |
dc.author.google | Seoh, Ju-Young | * |
dc.author.google | Lee, Ji Soo | * |
dc.author.google | Lee, Soon Nam | * |
dc.author.google | Kang, Jihee Lee | * |
dc.author.google | Choi, Youn-Hee | * |
dc.contributor.scopusid | 서주영(6603709174;57209001625) | * |
dc.contributor.scopusid | 이지희(7404517577) | * |
dc.contributor.scopusid | 이순남(8422735900;56949372000) | * |
dc.contributor.scopusid | 이지수(14424388700) | * |
dc.contributor.scopusid | 최윤희(7404776849) | * |
dc.date.modifydate | 20240123124738 | * |