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Determinants of Histone H3K4 Methylation Patterns

Title
Determinants of Histone H3K4 Methylation Patterns
Authors
Soares L.M.He P.C.Chun Y.Suh H.Kim T.Buratowski S.
Ewha Authors
김태수
SCOPUS Author ID
김태수scopus
Issue Date
2017
Journal Title
Molecular Cell
ISSN
1097-2765JCR Link
Citation
vol. 68, no. 4, pp. 773 - 7.85E08
Keywords
COMPASShistone H3 lysine 4histone methylationRNA polymerase IISet1transcription elongation
Publisher
Cell Press
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Various factors differentially recognize trimethylated histone H3 lysine 4 (H3K4me3) near promoters, H3K4me2 just downstream, and promoter-distal H3K4me1 to modulate gene expression. This methylation “gradient” is thought to result from preferential binding of the H3K4 methyltransferase Set1/complex associated with Set1 (COMPASS) to promoter-proximal RNA polymerase II. However, other studies have suggested that location-specific cues allosterically activate Set1. Chromatin immunoprecipitation sequencing (ChIP-seq) experiments show that H3K4 methylation patterns on active genes are not universal or fixed and change in response to both transcription elongation rate and frequency as well as reduced COMPASS activity. Fusing Set1 to RNA polymerase II results in H3K4me2 throughout transcribed regions and similarly extended H3K4me3 on highly transcribed genes. Tethered Set1 still requires histone H2B ubiquitylation for activity. These results show that higher-level methylations reflect not only Set1/COMPASS recruitment but also multiple rounds of transcription. This model provides a simple explanation for non-canonical methylation patterns at some loci or in certain COMPASS mutants. Soares et al. show that the H3K4 methylation gradient, an important chromatin modification at active genes, is determined not only by targeted recruitment of the Set1 methyltransferase but also by transcription frequency and elongation rate. Fusing Set1 to RNA polymerase results in extended H3K4 methylation throughout the gene. © 2017 Elsevier Inc.
DOI
10.1016/j.molcel.2017.10.013
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자연과학대학 > 생명과학전공 > Journal papers
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