Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 오억수 | - |
dc.date.accessioned | 2017-12-27T16:30:28Z | - |
dc.date.available | 2017-12-27T16:30:28Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 0264-6021 | - |
dc.identifier.other | OAK-21583 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/239313 | - |
dc.description.abstract | Syndecans (SDCs) are transmembrane proteoglycans that are involved in cell adhesion and cell communication. Specifically, SDC2 plays a key role in tumorigenesis, metastasis, and angiogenesis. Previously, we found that rat SDC2 is shed by matrix metalloproteinase-7 (MMP-7) in colon cancer cells. Here, we analyzed the susceptibility of rat SDC2 to various MMPs. We found that the rat SDC2 ectodomain (ECD) fused to the C-terminal Fc region, which was expressed in mammalian cells, was cleaved more efficiently by MMP-14 than MMP-7. Likewise, when anchored on the surface of HeLa cells, rat SDC2 was cleaved more efficiently by the treatment of MMP-14 than MMP-7 and was shed more readily by membrane-anchored MMP-14 than soluble MMP-14. Furthermore, MMP-14 cleaved recombinant SDC2-ECD expressed in Escherichia coli into multiple fragments. Using N-terminal amino acid sequencing and the top-down proteomics approach, we determined that the major cleavage sites were S88↓L89, T98↓M99, T100↓L101, D132↓P133, and N148↓L149 for rat SDC2-ECD and S55↓G56, S65↓P66, P75↓K76, N92↓I93 D122↓P123, and S138↓L139 for human SDC2-ECD. Finally, the rat and human SDC2-ECD lost the ability to suppress vascular endothelial growth factor-induced formation of capillary-like tubes by human umbilical vein endothelial cells following cleavage by MMP-14, but its major cleavage-site mutant of rat SDC2-ECD did not. These results suggest that MMP-14 is a novel enzyme responsible for degrading SDC2 and impairing its physiological roles including angiogenesis. © 2017 The Author(s). | - |
dc.language | English | - |
dc.publisher | Portland Press Ltd | - |
dc.title | Processing of syndecan-2 by matrix metalloproteinase-14 and effect of its cleavage on VEGF-induced tube formation of HUVECs | - |
dc.type | Article | - |
dc.relation.issue | 22 | - |
dc.relation.volume | 474 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 3719 | - |
dc.relation.lastpage | 3732 | - |
dc.relation.journaltitle | Biochemical Journal | - |
dc.identifier.doi | 10.1042/BCJ20170340 | - |
dc.identifier.wosid | WOS:000415328400002 | - |
dc.identifier.scopusid | 2-s2.0-85033379773 | - |
dc.author.google | Lee Y.H. | - |
dc.author.google | Park J.H. | - |
dc.author.google | Cheon D.H. | - |
dc.author.google | Kim T. | - |
dc.author.google | Park Y.E. | - |
dc.author.google | Oh E.-S. | - |
dc.author.google | Lee J.E. | - |
dc.author.google | Lee S.-T. | - |
dc.contributor.scopusid | 오억수(7101967153) | - |
dc.date.modifydate | 20230201093717 | - |