View : 24 Download: 0

Generation of ROS by IgE-dependent histamine-releasing factor in RBL-2H3 cells

Title
Generation of ROS by IgE-dependent histamine-releasing factor in RBL-2H3 cells
Authors
Choo Y.S.Lee K.
Ewha Authors
이경림
SCOPUS Author ID
이경림scopus
Issue Date
2005
Journal Title
Korean Journal of Microbiology and Biotechnology
ISSN
1598-642XJCR Link
Citation
vol. 33, no. 3, pp. 231 - 235
Indexed
SCOPUS; KCI scopus
Abstract
Histamine-releasing factors (HRFs) are soluble mediators that can release histamine and other mediators from basophils and mast cells and their activity can vary, depending on the type of IgE. The activity of HRFs is affected by the presence of IgE, although HRF is thought to bind to a specific receptor other than IgE. Until now, HRF signaling pathway including its receptor remains unclear in spite of numerous studies. Since there had been many reports about reactive oxygen species (ROS) as a signaling molecule rather than as a by-product of metabolism, we investigated the possibility of ROS as an intracellular messenger involved in HRF-mediated histamine degranulation. In RBL-2H3 cells, ROS was generated by HRF using H2O2- sensitive fluorescence of fluorescent 2′, 7′-dichlorofluorescein (H2DCFDA). These effects were blocked by anti-oxidant N-acetylcysteine (NAC). These results suggest that ROS generation could play a role as an intracellular messenger in histamine release by HRF.
Appears in Collections:
약학대학 > 약학과 > Journal papers
Files in This Item:
There are no files associated with this item.


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE