Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 손형진 | - |
dc.contributor.author | 신동해 | - |
dc.date.accessioned | 2017-11-01T05:01:52Z | - |
dc.date.available | 2017-11-01T05:01:52Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.other | OAK-21080 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/239045 | - |
dc.description.abstract | Defective hepatic autophagy is observed in obesity and diabetes, whereas autophagy is inhibited by insulin in hepatocytes. Insulin-induced anti-autophagy is mediated by non-canonical Gαi3 signaling via an unknown mechanism. Previously, we identified the anti-autophagic activity of Tnfaip8 via activation of mammalian target of rapamycin (mTOR) in the nervous system. Here, we demonstrate that insulin temporally induces Tnfaip8, which mediates the anti-autophagic action of insulin through formation of a novel ternary complex including Tnfaip8, phosphatidylethanolamine (PE) and Gαi3. Specifically, an X-ray crystallographic study of Tnfaip8 from Mus musculus (mTnfaip8) at 2.03 Å together with LC-MS analyses reveals PE in the hydrophobic cavity. However, an mTnfaip8 mutant lacking PE does not interact with Gαi3, indicating that the PE component is critical for the anti-autophagic action of mTnfaip8 via interaction with Gαi3. Therefore, the mTnfaip8-PE complex may act as an essential upstream effector via ternary complex formation most likely with active Gαi3 during insulin-induced anti-autophagy. © 2017 The Author(s). | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.title | The Tnfaip8-PE complex is a novel upstream effector in the anti-autophagic action of insulin | - |
dc.type | Article | - |
dc.relation.issue | 1 | - |
dc.relation.volume | 7 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.journaltitle | Scientific Reports | - |
dc.identifier.doi | 10.1038/s41598-017-06576-3 | - |
dc.identifier.wosid | WOS:000406260100052 | - |
dc.identifier.scopusid | 2-s2.0-85025818778 | - |
dc.author.google | Kim J.-S. | - |
dc.author.google | Park J. | - |
dc.author.google | Kim M.-S. | - |
dc.author.google | Ha J.-Y. | - |
dc.author.google | Jang Y.-W. | - |
dc.author.google | Shin D.H. | - |
dc.author.google | Son J.H. | - |
dc.contributor.scopusid | 손형진(7203086503) | - |
dc.contributor.scopusid | 신동해(57217374185) | - |
dc.date.modifydate | 20230208115524 | - |