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Bioprocess engineering to produce 9-(nonanoyloxy) nonanoic acid by a recombinant Corynebacterium glutamicum-based biocatalyst

Title
Bioprocess engineering to produce 9-(nonanoyloxy) nonanoic acid by a recombinant Corynebacterium glutamicum-based biocatalyst
Authors
Kim H.Park S.Cho S.Yang J.Jeong K.Park J.Lee J.
Ewha Authors
박진병
SCOPUS Author ID
박진병scopus
Issue Date
2017
Journal Title
Journal of Industrial Microbiology and Biotechnology
ISSN
1367-5435JCR Link
Citation
vol. 44, no. 9, pp. 1301 - 1311
Keywords
10-Ketostearic acid9-(Nonanoyloxy) nonanoic acidBiocatalystCorynebacterium glutamicum
Publisher
Springer Verlag
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Here, Corynebacterium glutamicum ATCC13032 expressing Baeyer–Villiger monooxygenase from Pseudomonas putida KT2440 was designed to produce 9-(nonanoyloxy) nonanoic acid from 10-ketostearic acid. Diverse parameters including cultivation and reaction temperatures, type of detergent, and pH were found to improve biotransformation efficiency. The optimal temperature of cultivation for the production of 9-(nonanoyloxy) nonanoic acid from 10-ketostearic acid using whole cells of recombinant C. glutamicum was 15 °C, but the reaction temperature was optimal at 30 °C. Enhanced conversion efficiency was obtained by supplying 0.05 g/L of Tween 80 at pH 7.5. Under these optimal conditions, recombinant C. glutamicum produced 0.28 mM of 9-(nonanoyloxy) nonanoic acid with a 75.6% (mol/mol) conversion yield in 2 h. This is the first report on the biotransformation of 10-ketostearic acid to 9-(nonanoyloxy) nonanoic acid with a recombinant whole-cell C. glutamicum-based biocatalyst and the results demonstrate the feasibility of using C. glutamicum as a whole-cell biocatalyst. © 2017, Society for Industrial Microbiology and Biotechnology.
DOI
10.1007/s10295-017-1945-9
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엘텍공과대학 > 식품공학전공 > Journal papers
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