View : 14 Download: 0

Identification of Secondary Metabolites from a Fungus Alternaria sp. from the plant Rheum Palmatum

Title
Identification of Secondary Metabolites from a Fungus Alternaria sp. from the plant Rheum Palmatum
Authors
김연지
Issue Date
2017
Department/Major
대학원 화학·나노과학과
Publisher
이화여자대학교 대학원
Degree
Master
Advisors
남상집
Abstract
Fungi have appeared growing irregularly in several species of plants. Such fungi was observed to complete most, even though it is not all, their life cycle within the plant, so they were commonly called Plant-associated fungus. Plant-associated fungus usually live asymptomatically in tissues of their host plants and inside vascular tissues without causing evident pathogenic symptoms. Growing evidence indicates that plant-associated fungus are found in all plants, are plentiful and are often quite diverse. In general most of plant-associated fungi form internal localized infections in leaves, roots, stems and bark and are horizontally carried via spores. In our research, two known compounds, alternariol monomethyl ether (1) and altertoxin Ⅱ (2), were isolated from Alternaria sp. strain SNF008 from Rheum Palmatum. Their chemical structures also were elucidated by LC-MS and NMR spectroscopy data (1H, 13C, COSY, HMBC and HSQC). The compounds were tested for the inhibitory activity of monoamine oxidase (MAO), which is known to catalyze neurotransmitting monoamines. AME was found to be a considerably influential and selective inhibitor of human MAO-A with an IC50 value of 1.71 µM; however, it was not found to be effective for MAO-B inhibition. ATXⅡ was ineffective for the inhibition of either MAO-A or MAO-B. From the sample obtained from AP, two known ginsenosides compound-K (1) and compound Y (2), were isolated. Their structures also were elucidated by LC-MS and NMR spectroscopy data (1H, 13C, COSY, HMBC and HSQC). ;다양한 식물에서 곰팡이는 식물의 세포 안 쪽에서 자라는 것이 발견 되어왔다. 식물의 내부에서 자라는 곰팡이는 숙주 식물 그 자체와 그것의 세포에 해를 입히거나 그 속에서 사는 미생물에게 도움을 주는 등의 영향을 주지 않는다. 식물유래 곰팡이는 주로 잎이나 뿌리, 줄기, 껍질들에서 만들어지며 포자의 형태로 이동한다. 식물유래 곰팡이의 흔적이 증가하는 것은 식물유래 곰팡이가 모든 식물에 존재하고 그 원천이 상당히 풍부하고 다양하다는 것을 의미하며 따라서 이것에 대해 관심 있게 연구할 필요성이 있다. 본 연구에서는 대황(Rheum Palmatum) 줄기에서 분리한 Alternaria sp. Strain인 SNF008에서 이미 알려진 두 가지 물질인 alternariol monomethyl ether (1) 과 altertoxin Ⅱ (2)을 분리하였고 LC-MS와 NMR (1H, 13C, COSY, HMBC, HSQC)를 이용하여 그들의 구조를 구명하였다. 이 두 가지 물질에 대한 MAO활성 실험을 추가적으로 진행했고 AME (1) 가 MAO-A에 대한 활성을 나타내는 것을 알 수 있었다. 반면, ATX II는 MAO-A와 MAO-B에 대한 활성이 모두 나타나지 않았다. AP로부터 받은 샘플로부터 알려진 물질인 ginsenoside compound-K (1) 와 ginsenoside compound-Y (2)를 분리정제하였으며 LC-MS와 NMR을 이용하여 구조를 구명하였다.
Fulltext
Show the fulltext
Appears in Collections:
일반대학원 > 화학·나노과학과 > Theses_Master
Files in This Item:
There are no files associated with this item.


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE