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Detection of bisphenol A using palm-size NanoAptamer analyzer

Title
Detection of bisphenol A using palm-size NanoAptamer analyzer
Authors
Lim H.J.Chua B.Son A.
Ewha Authors
손아정
SCOPUS Author ID
손아정scopus
Issue Date
2017
Journal Title
Biosensors and Bioelectronics
ISSN
0956-5663JCR Link
Citation
vol. 94, pp. 10 - 18
Keywords
AptamerBisphenol A (BPA)Fluorescence measurementMagnetic beadNanoAptamer analyzerQuantum dots
Publisher
Elsevier Ltd
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
We have demonstrated a palm-size NanoAptamer analyzer capable of detecting bisphenol A (BPA) at environmentally relevant concentrations (<1 ng/mL or ppb). It is designed for performing reaction and fluorescence measurement on single cuvette sample. Modified NanoGene assay was used as the sensing mechanism where signaling DNA and QD655 was tethered to QD565 and magnetic bead via the aptamer. Aptamer affinity with BPA resulted in the release of the signaling DNA and QD655 from the complex and hence corresponding decrease in QD655 fluorescence measurement signal. Baseline characterization was first performed with empty cuvettes, quantum dots and magnetic beads under near-ideal conditions to establish essential functionality of the NanoAptamer analyzer. Duration of incubation time, number of rinse cycles, and necessity of cuvette vibration were also investigated. In order to demonstrate the capability of the NanoAptamer analyzer to detect BPA, samples with BPA concentrations ranging from 0.0005 to 1.0 ng/mL (ppb) were used. The performance of the NanoAptamer analyzer was further examined by using laboratory protocol and commercial spectrofluorometer as reference. Correlation between NanoAptamer analyzer and laboratory protocol as well as commercial spectrofluorometer was evaluated via correlation plots and correlation coefficients. © 2017 Elsevier B.V.
DOI
10.1016/j.bios.2017.02.029
Appears in Collections:
엘텍공과대학 > 환경공학전공 > Journal papers
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